BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 1581

Title: Deletion of the omega-loop in the Active Site of Staphylococcal Nuclease 2. Effects on Protein Structure and Dynamics

Authors: Baldisseri, Donna; Torchia, Dennis; Poole, Leslie; Gerlt, John

Citation: Baldisseri, Donna; Torchia, Dennis; Poole, Leslie; Gerlt, John. "Deletion of the omega-loop in the Active Site of Staphylococcal Nuclease 2. Effects on Protein Structure and Dynamics"  Biochemistry 30, 3628-3633 (1991).

Assembly members:
micrococcal nuclease, polymer, 156 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: not available   Host organism: Escherichia coli

Entity Sequences (FASTA):
micrococcal nuclease: XXXXXXXXXXXXKLHKEPAT LIKAIDGDTVKLMYKGQPMT FRLLLVDTPETKHPKKGVEK YGPEASAFTKKMVENAKKIE VEFNKGQRTDKYGRGLAYIY ADGKMVNEALVRQGLAKVAY VYKPNNTHEQHLRKSEAQAK KEKLNIWSENDADSGQ

Data sets:
Data typeCount
15N chemical shifts135
1H chemical shifts135

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1micrococcal nuclease1

Entities:

Entity 1, micrococcal nuclease 156 residues - Formula weight is not available

1   XXXXXXXXXX
2   XXLYSLEUHISLYSGLUPROALATHR
3   LEUILELYSALAILEASPGLYASPTHRVAL
4   LYSLEUMETTYRLYSGLYGLNPROMETTHR
5   PHEARGLEULEULEUVALASPTHRPROGLU
6   THRLYSHISPROLYSLYSGLYVALGLULYS
7   TYRGLYPROGLUALASERALAPHETHRLYS
8   LYSMETVALGLUASNALALYSLYSILEGLU
9   VALGLUPHEASNLYSGLYGLNARGTHRASP
10   LYSTYRGLYARGGLYLEUALATYRILETYR
11   ALAASPGLYLYSMETVALASNGLUALALEU
12   VALARGGLNGLYLEUALALYSVALALATYR
13   VALTYRLYSPROASNASNTHRHISGLUGLN
14   HISLEUARGLYSSERGLUALAGLNALALYS
15   LYSGLULYSLEUASNILETRPSERGLUASN
16   ASPALAASPSERGLYGLN

Samples:

sample_one:

sample_condition_set_one: pH: 6.4 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 136 1582 16585 1704 17718 1874 1875 1876 1877 1878 188 189 2784 2785 4010 4052 4053
PDB
DBJ BAB94634 BAF67032 BAR08312 BAR11036 BAS50937
EMBL CAA24594 CAG39855 CAG42530 CAQ49298 CBI48745
GB AAC14660 AAW36415 ABD22328 ABD29945 ABF58092
PRF 1109959A 710414A
REF WP_000141556 WP_001548082 WP_001566557 WP_001574556 WP_001641381
SP P00644 Q5HHM4 Q6GB41 Q6GIK1 Q8NXI6