BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 1657

Title: Assignments of Backbone 1H, 13C, 15N Resonances and Secondary Structure of Ribonuclease H from Escherichia coli by Heteronuclear Three-Dimensional NMR Spectroscopy

Authors: Yamazaki, Toshio; Yoshida, Mayumi; Kanaya, Shigenori; Nakamura, Haruki; Nagayama, Kuniaki

Citation: Yamazaki, Toshio; Yoshida, Mayumi; Kanaya, Shigenori; Nakamura, Haruki; Nagayama, Kuniaki. "Assignments of Backbone 1H, 13C, 15N Resonances and Secondary Structure of Ribonuclease H from Escherichia coli by Heteronuclear Three-Dimensional NMR Spectroscopy"  Biochemistry 30, 6036-6047 (1991).

Assembly members:
ribonuclease H, polymer, 155 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: not available   Host organism: Escherichia coli

Entity Sequences (FASTA):
ribonuclease H: MLKQVEIFTDGSCLGNPGPG GYGAILRYRGREKTFSAGYT RTTNNRMELMAAIVALEALK EHCEVILSTDSQYVRQGITQ WIHNWKKRGWKTADKKPVKN VDLWQRLDAALGQHQIKWEW VKGHAGHPENERCDELARAA AMNPTLEDTGYQVEV

Data sets:
Data typeCount
13C chemical shifts122
15N chemical shifts148
1H chemical shifts317

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ribonuclease H1

Entities:

Entity 1, ribonuclease H 155 residues - Formula weight is not available

1   METLEULYSGLNVALGLUILEPHETHRASP
2   GLYSERCYSLEUGLYASNPROGLYPROGLY
3   GLYTYRGLYALAILELEUARGTYRARGGLY
4   ARGGLULYSTHRPHESERALAGLYTYRTHR
5   ARGTHRTHRASNASNARGMETGLULEUMET
6   ALAALAILEVALALALEUGLUALALEULYS
7   GLUHISCYSGLUVALILELEUSERTHRASP
8   SERGLNTYRVALARGGLNGLYILETHRGLN
9   TRPILEHISASNTRPLYSLYSARGGLYTRP
10   LYSTHRALAASPLYSLYSPROVALLYSASN
11   VALASPLEUTRPGLNARGLEUASPALAALA
12   LEUGLYGLNHISGLNILELYSTRPGLUTRP
13   VALLYSGLYHISALAGLYHISPROGLUASN
14   GLUARGCYSASPGLULEUALAARGALAALA
15   ALAMETASNPROTHRLEUGLUASPTHRGLY
16   TYRGLNVALGLUVAL

Samples:

sample_one:

sample_condition_set_one: pH: 5.5 na; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 4012
PDB
DBJ BAA77885 BAB33633 BAG75734 BAI23570 BAI29084
EMBL CAA23620 CAA27660 CAP74778 CAQ30729 CAQ87812
GB AAA24565 AAB08636 AAC73319 AAG54510 AAN41862
REF NP_308237 NP_414750 NP_706155 WP_000917867 WP_000917875
SP A7ZHV1 A7ZWF6 B1IPU4 B1LHM3 B1XD78