BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 1658

Title: Two-dimensional 1H, 15N-NMR investigation of uniformly 15N-labeled ribonuclease T1 (Complete assignment of 15N resonances)

Authors: Schmidt, Jurgen; Thuring, Harald; Werner, Angela; Ruterjans, Heinz; Quaas, Rainer; Hahn, Ulrich

Citation: Schmidt, Jurgen; Thuring, Harald; Werner, Angela; Ruterjans, Heinz; Quaas, Rainer; Hahn, Ulrich. "Two-dimensional 1H, 15N-NMR investigation of uniformly 15N-labeled ribonuclease T1 (Complete assignment of 15N resonances)"  Eur. J. Biochem. 197, 643-653 (1991).

Assembly members:
ribonuclease T1, polymer, 104 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 5062   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Aspergillus oryzae

Experimental source:   Production method: not available   Host organism: Escherichia coli

Entity Sequences (FASTA):
ribonuclease T1: ACDYTCGSNCYSSSDVSTAQ AAGYKLHEDGETVGSNSYPH KYNNYEGFDFSVSSPYYEWP ILSSGDVYSGGSPGADRVVF NENNQLAGVITHTGASGNNF VECT

Data sets:
Data typeCount
15N chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ribonuclease T11

Entities:

Entity 1, ribonuclease T1 104 residues - Formula weight is not available

1   ALACYSASPTYRTHRCYSGLYSERASNCYS
2   TYRSERSERSERASPVALSERTHRALAGLN
3   ALAALAGLYTYRLYSLEUHISGLUASPGLY
4   GLUTHRVALGLYSERASNSERTYRPROHIS
5   LYSTYRASNASNTYRGLUGLYPHEASPPHE
6   SERVALSERSERPROTYRTYRGLUTRPPRO
7   ILELEUSERSERGLYASPVALTYRSERGLY
8   GLYSERPROGLYALAASPARGVALVALPHE
9   ASNGLUASNASNGLNLEUALAGLYVALILE
10   THRHISTHRGLYALASERGLYASNASNPHE
11   VALGLUCYSTHR

Samples:

sample_one:

sample_condition_set_one: pH: 5.5 na; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 15905 16469 16580
PDB
DBJ BAA05707 BAA08407 BAE64671
EMBL CAA30560
GB AAA72541 AAA72994 EED52238 EIT74451 KDE77875
REF XP_001825804 XP_002377402
SP P00651