BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4019

Title: 1H, 13C and 15N Resonance Assignments of Recombinant Poplar Plastocyanin

Authors: Koide, Shohei; Gippert, Garry; Reymond, Martine; Wright, Peter

Citation: Koide, Shohei; Gippert, Garry; Reymond, Martine; Wright, Peter. "1H, 13C and 15N Resonance Assignments of Recombinant Poplar Plastocyanin"  The BMRB entry is the only known published source for the data..

Assembly members:
plastocyanin peptide, polymer, 99 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.

Natural source:   Common Name: poplar   Taxonomy ID: 3697   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Populus populus sp.

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
plastocyanin peptide: IDVLLGADDGSLAFVPSEFS ISPGEKIVFKNNAGFPHNIV FDEDSIPSGVDASKISMSEE DLLNAKGETFEVALSNKGEY SFYCSPHQGAGMVGKVTVN

Data typeCount
13C chemical shifts425
15N chemical shifts101
1H chemical shifts668

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pc peptide1
2CuI2

Entities:

Entity 1, Pc peptide 99 residues - Formula weight is not available

1   ILEASPVALLEULEUGLYALAASPASPGLY
2   SERLEUALAPHEVALPROSERGLUPHESER
3   ILESERPROGLYGLULYSILEVALPHELYS
4   ASNASNALAGLYPHEPROHISASNILEVAL
5   PHEASPGLUASPSERILEPROSERGLYVAL
6   ASPALASERLYSILESERMETSERGLUGLU
7   ASPLEULEUASNALALYSGLYGLUTHRPHE
8   GLUVALALALEUSERASNLYSGLYGLUTYR
9   SERPHETYRCYSSERPROHISGLNGLYALA
10   GLYMETVALGLYLYSVALTHRVALASN

Entity 2, CuI - Cu - 63.546 Da.

1   CU1

Samples:

sample_one: plastocyanin, none,

sample_two: plastocyanin, [15N-U],

sample_three: plastocyanin, [13C-U; 15N-U],

reporting_conditions_set_one: pH: 7 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablereporting_conditions_set_one

Software:

FELIX, MSI/BIOSYM, San Diego - Peak picking, Peak volume integration

GENMAC, . - Generate FELIX macros for NH strip manipulation

GENXPK, . - Assign new peaks, Automatically generate complete sets of crosspeak footprints, Compare homologous chemical shifts from different protein species, Monitor the relocation of footprints to new positions

NMR spectrometers:

    Related Database Links:

    BMRB 19247
    PDB
    EMBL CAA90564
    GB ABK96770 EEE94750
    REF XP_002307754 XP_011003094
    SP P00299