BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4022

Title: Sequential NMR Assignment of 1H, 13C, and 15N Resonances of Human Carbonic Anhydrase I (HCAI) by Triple-Resonance NMR Techniques and Extensive Amino Acid Specific 15N Labeling

Authors: Sethson, Ingmar; Edlund, Ulf; Holak, Tadeusz; Ross, Alfred; Jonsson, Bengt-Harald

Citation: Sethson, Ingmar; Edlund, Ulf; Holak, Tadeusz; Ross, Alfred; Jonsson, Bengt-Harald. "Sequential Assignment of 1H, 13C and 15N Resonances of Human Carbonic Anhydrase I by Triple-Resonance NMR Techniques and Extensive Amino Acid-Specific 15N-Labeling"  J. Biomol. NMR 8, 417-428 (1996).

Assembly members:
human carbonic anhydrase I, polymer, 260 residues, 29500 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
human carbonic anhydrase I: ASPDWGYDDKNGPEQWSKLY PIANGNNQSPVDIKTSETKH DTSLKPISVSYNPATAKEII NVGHSFHVNFEDNDNRSVLK GGPFSDSYRLFQFHFHWGST NEHGSEHTVDGVKYSAELHV AHWNSAKYSSLAEAASKADG LAVIGVLMKVGEANPKLQKV LDALQAIKTKGKRAPFTNFD PSTLLPSSLDFWTYPGSLTH PPLYESVTWIICKESISVSS EQLAQFRSLLSNVEGDNAVP MQHNNRPTQPLKGRTVRASF

Data sets:
Data typeCount
13C chemical shifts743
15N chemical shifts243
1H chemical shifts861

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HCA I1

Entities:

Entity 1, HCA I 260 residues - 29500 Da.

1   ALASERPROASPTRPGLYTYRASPASPLYS
2   ASNGLYPROGLUGLNTRPSERLYSLEUTYR
3   PROILEALAASNGLYASNASNGLNSERPRO
4   VALASPILELYSTHRSERGLUTHRLYSHIS
5   ASPTHRSERLEULYSPROILESERVALSER
6   TYRASNPROALATHRALALYSGLUILEILE
7   ASNVALGLYHISSERPHEHISVALASNPHE
8   GLUASPASNASPASNARGSERVALLEULYS
9   GLYGLYPROPHESERASPSERTYRARGLEU
10   PHEGLNPHEHISPHEHISTRPGLYSERTHR
11   ASNGLUHISGLYSERGLUHISTHRVALASP
12   GLYVALLYSTYRSERALAGLULEUHISVAL
13   ALAHISTRPASNSERALALYSTYRSERSER
14   LEUALAGLUALAALASERLYSALAASPGLY
15   LEUALAVALILEGLYVALLEUMETLYSVAL
16   GLYGLUALAASNPROLYSLEUGLNLYSVAL
17   LEUASPALALEUGLNALAILELYSTHRLYS
18   GLYLYSARGALAPROPHETHRASNPHEASP
19   PROSERTHRLEULEUPROSERSERLEUASP
20   PHETRPTHRTYRPROGLYSERLEUTHRHIS
21   PROPROLEUTYRGLUSERVALTHRTRPILE
22   ILECYSLYSGLUSERILESERVALSERSER
23   GLUGLNLEUALAGLNPHEARGSERLEULEU
24   SERASNVALGLUGLYASPASNALAVALPRO
25   METGLNHISASNASNARGPROTHRGLNPRO
26   LEULYSGLYARGTHRVALARGALASERPHE

Samples:

sample_one: human carbonic anhydrase I, [U-15N], 0.8 mM; D2O 5%; H2O 95%; phosphate buffer 40 mM

sample_two: human carbonic anhydrase I, [U-13C], 0.8 mM; D2O 5%; H2O 95%; phosphate buffer 40 mM

sample_three: human carbonic anhydrase I, [15N]-Ala;[15N]-Gly;[15N]-His;[15N]-Ile;[15N]-Leu;[15N]-Lys;[15N]-Phe; [15N]-Ser;[15N]-Trp;[15N]-Val , 5; 4022, 3, 95; 4022, 3, 40; 4022, 3,

sample_conditions_one: pH: 6.2 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
EMBL CAA28663
GB AAA51910 AAH27890 ABM81856 ABM85017 ACJ13649
REF NP_001122301 NP_001122302 NP_001122303 NP_001158302 NP_001182062
SP P00915 Q7M316 Q7M317