BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4028

Title: Chemical Shift Mapping of the RNA-binding Interface to the Multiple-RBD Protein Sex-Lethal: Chemical Shifts for the Protein-RNA Complex

Authors: Lee, Andrew; Volkman, Brian; Robertson, Stephanie; Rudner, David; Barbash, Daniel; Cline, Thomas; Kanaar, Roland; Rio, Donald; Wemmer, David

Citation: Lee, Andrew; Volkman, Brian; Robertson, Stephanie; Rudner, David; Barbash, Daniel; Cline, Thomas; Kanaar, Roland; Rio, Donald; Wemmer, David. "Chemical Shift Mapping of the RNA-binding Interface to the Multiple-RBD Protein Sex-Lethal"  Biochemistry 36, 14306-14317 (1997).

Assembly members:
Sex-lethal protein, polymer, 184 residues, Formula weight is not available
polypyrimidine tract 10-mer RNA, polymer, 10 residues, Formula weight is not available

Natural source:   Common Name: fruitfly   Taxonomy ID: 7215   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Sex-lethal protein: MGSDDLMNDPRASNTNLIVN YLPQDMTDRELYALFRAIGP INTCRIMRDYKTGYSFGYAF VDFTSEMDSQRAIKVLNGIT VRNKRLKVSYARPGGESIKD TNLYVTNLPRTITDDQLDTI FGKYGSIVQKNILRDKLTGR PRGVAFVRYNKREEAQEAIS ALNNVIPEGGSQPLSVRLAE EHGK
polypyrimidine tract 10-mer RNA: GUUUUUUUUC

Data sets:
Data typeCount
13C chemical shifts308
15N chemical shifts149
1H chemical shifts261

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sex-lethal protein1
2RNA2

Entities:

Entity 1, Sex-lethal protein 184 residues - Formula weight is not available

1   METGLYSERASPASPLEUMETASNASPPRO
2   ARGALASERASNTHRASNLEUILEVALASN
3   TYRLEUPROGLNASPMETTHRASPARGGLU
4   LEUTYRALALEUPHEARGALAILEGLYPRO
5   ILEASNTHRCYSARGILEMETARGASPTYR
6   LYSTHRGLYTYRSERPHEGLYTYRALAPHE
7   VALASPPHETHRSERGLUMETASPSERGLN
8   ARGALAILELYSVALLEUASNGLYILETHR
9   VALARGASNLYSARGLEULYSVALSERTYR
10   ALAARGPROGLYGLYGLUSERILELYSASP
11   THRASNLEUTYRVALTHRASNLEUPROARG
12   THRILETHRASPASPGLNLEUASPTHRILE
13   PHEGLYLYSTYRGLYSERILEVALGLNLYS
14   ASNILELEUARGASPLYSLEUTHRGLYARG
15   PROARGGLYVALALAPHEVALARGTYRASN
16   LYSARGGLUGLUALAGLNGLUALAILESER
17   ALALEUASNASNVALILEPROGLUGLYGLY
18   SERGLNPROLEUSERVALARGLEUALAGLU
19   GLUHISGLYLYS

Entity 2, RNA 10 residues - Formula weight is not available

1   GUUUUUUUUC

Samples:

sample_one: Sex-lethal protein, [U-95% 13C; U-95% 15N], 0.5 – 1.0 mM; polypyrimidine tract 10-mer RNA0.5 – 1.0 mM; H2O 90%; D2O 10%; NaCl 30 mM; NaN3 0.02%; sodium phosphate buffer 30 mM

sample_condition_one: pH: 6.30 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions

Software:

xwinnmr v1.1, Bruker - NMR data acquisition

FELIX v2.30, biosym - fourier transformation, peak picking, strip plotting, visualization

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

BMRB 4029
PDB
GB AAA28884 AAA28921 AAA28922 AAF46240 AAF46241
PIR B39725
REF NP_001027062 NP_001027063 NP_001027065 NP_001162686 NP_001162688
SP P19339