BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4045

Title: Solution Structure of the C-Terminal Single-Stranded DNA-Binding Domain of Escherichia Coli Topoisomerase I

Authors: Yu, Liping; Zhu, Chang-Xi; Tse-Dinh, Yuk-Ching; Fesik, Stephen

Citation: Yu, Liping; Zhu, Chang-Xi; Tse-Dinh, Yuk-Ching; Fesik, Stephen. "Solution Structure of the C-Terminal Single-Stranded DNA-Binding Domain of Escherichia coli Topoisomerase I"  Biochemistry 34, 7622-7628 (1995).

Assembly members:
DNA Topoisomerase I, polymer, 122 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DNA Topoisomerase I: MNGEVAPPKEDPVPLPELPC EKSDAYFVLRDGAAGVFLAA NTFPKSRETRAPLVEELYRF RDRLPEKLRYLADAPQQDPE GNKTMVRFSRKTKQQYVSSE KDGKATGWSAFYVDGKWVEG KK

Data sets:
Data typeCount
13C chemical shifts441
15N chemical shifts110
1H chemical shifts849

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA Topoisomerase I1

Entities:

Entity 1, DNA Topoisomerase I 122 residues - Formula weight is not available

1   METASNGLYGLUVALALAPROPROLYSGLU
2   ASPPROVALPROLEUPROGLULEUPROCYS
3   GLULYSSERASPALATYRPHEVALLEUARG
4   ASPGLYALAALAGLYVALPHELEUALAALA
5   ASNTHRPHEPROLYSSERARGGLUTHRARG
6   ALAPROLEUVALGLUGLULEUTYRARGPHE
7   ARGASPARGLEUPROGLULYSLEUARGTYR
8   LEUALAASPALAPROGLNGLNASPPROGLU
9   GLYASNLYSTHRMETVALARGPHESERARG
10   LYSTHRLYSGLNGLNTYRVALSERSERGLU
11   LYSASPGLYLYSALATHRGLYTRPSERALA
12   PHETYRVALASPGLYLYSTRPVALGLUGLY
13   LYSLYS

Samples:

sample_one: DNA Topoisomerase I, [U-15N], 3 mM; dithiothreitol-d10 5 mM; KH2PO4 200 mM

sample_two: DNA Topoisomerase I, [U-13C; U-15N], 3 mM; dithiothreitol-d10 5 mM; KH2PO4 200 mM

sample_conditions_one: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

PDB
DBJ BAA14811 BAB35269 BAG76846 BAI25122 BAI30265
EMBL CAA28164 CAP75818 CAQ31777 CAQ98153 CAR02734
GB AAA23641 AAC74356 AAG56541 AAN42888 AAN80207
REF NP_309873 NP_415790 NP_707181 WP_000317025 WP_000513607
SP P06612