BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4046

Title: NMR Evidence for Similarities between the DNA-Binding Regions of Drosophila melanogaster Heat Shock Factor and the Helix-Turn-Helix and HNF-3/forkhead Families of Transcription Factors

Authors: Vuister, Geerten; Kim, Soon-Jong; Wu, Carl; Bax, Ad

Citation: Vuister, Geerten; Kim, Soon-Jong; Wu, Carl; Bax, Ad. "NMR Evidence for Similarities between the DNA-Binding Regions of Drosophila melanogaster Heat Shock Factor and the Helix-Turn-Helix and HNF-3/forkhead Families of Transcription Factors"  . 33, 10-16 (1994).

Assembly members:
Drosophila Heat Shock Factor, polymer, 136 residues, 15259 Da.

Natural source:   Common Name: Fruit Fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Drosophila Heat Shock Factor: AAEHSYGDAAAIGSGVPAFL AKLWRIVDDADTNRLICWTK DGQSFVIQNQAQFAKELLPL NYKHNNMASFIRQLNMYGFH KITSIDNGGLRFDRDEIEFS HPFFKRNSPFLLDQIKRKIS NNKNGDDKGVLKPAAE

Data sets:
Data typeCount
13C chemical shifts370
15N chemical shifts122
1H chemical shifts454

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1dHSF1

Entities:

Entity 1, dHSF 136 residues - 15259 Da.

1   ALAALAGLUHISSERTYRGLYASPALAALA
2   ALAILEGLYSERGLYVALPROALAPHELEU
3   ALALYSLEUTRPARGILEVALASPASPALA
4   ASPTHRASNARGLEUILECYSTRPTHRLYS
5   ASPGLYGLNSERPHEVALILEGLNASNGLN
6   ALAGLNPHEALALYSGLULEULEUPROLEU
7   ASNTYRLYSHISASNASNMETALASERPHE
8   ILEARGGLNLEUASNMETTYRGLYPHEHIS
9   LYSILETHRSERILEASPASNGLYGLYLEU
10   ARGPHEASPARGASPGLUILEGLUPHESER
11   HISPROPHEPHELYSARGASNSERPROPHE
12   LEULEUASPGLNILELYSARGLYSILESER
13   ASNASNLYSASNGLYASPASPLYSGLYVAL
14   LEULYSPROALAALAGLU

Samples:

sample_one: Drosophila Heat Shock Factor, [U-15N], 0.7 – 1.1 mM; D2O 5%; potassium phosphate 10 mM; KCl 50 mM

sample_two: Drosophila Heat Shock Factor, [U-13C; U-15N], 0.7 – 1.1 mM; D2O 5%; potassium phosphate 10 mM; KCl 50 mM

sample_conditions_one: pH: 6.3 na; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAE16321 BAE16322 BAE16323
GB AAA28642 AAF57749 AAL13990 ABI31102 ABI31103
REF NP_001036555 NP_001036556 NP_001036557 NP_476575 XP_002034392
SP P22813