BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4049

Title: Solution Structure of the Amino-Terminal Fragment of Urokinase-Type Plasminogen Activator

Authors: Hansen, Andrew; Petros, Andrew; Meadows, Robert; Nettesheim, David; Mazar, Andrew; Olejniczak, Edward; Xu, Robert; Pederson, Terry; Henkin, Jack; Fesik, Stephen

Citation: Hansen, Andrew; Petros, Andrew; Meadows, Robert; Nettesheim, David; Mazar, Andrew; Olejniczak, Edward; Xu, Robert; Pederson, Terry; Henkin, Jack; Fesik, Stephen. "Solution Structure of the Amino-Terminal Fragment of Urokinase-Type Plasminogen Activator"  Biochemistry 33, 4847-4864 (1994).

Assembly members:
u-PA amino-terminal fragment, polymer, 130 residues, Formula weight is not available
BETA-D-FUCOSE, non-polymer, 164.156 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
u-PA amino-terminal fragment: QVPSNCDCLNGGTCVSNKYF SNIHWCNCPKKFGGQHCEID KSKTCYEGNGHFYRGKASTD TMGRPCLPWNSATVLQQTYH AHRSDALQLGLGKHNYCRNP DNRRRPWCYVQVGLKPLVQE CMVHDCADGK

Data sets:
Data typeCount
13C chemical shifts398
15N chemical shifts122
1H chemical shifts822

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ATF1

Entities:

Entity 1, ATF 130 residues - Formula weight is not available

1   GLNVALPROSERASNCYSASPCYSLEUASN
2   GLYGLYTHRCYSVALSERASNLYSTYRPHE
3   SERASNILEHISTRPCYSASNCYSPROLYS
4   LYSPHEGLYGLYGLNHISCYSGLUILEASP
5   LYSSERLYSTHRCYSTYRGLUGLYASNGLY
6   HISPHETYRARGGLYLYSALASERTHRASP
7   THRMETGLYARGPROCYSLEUPROTRPASN
8   SERALATHRVALLEUGLNGLNTHRTYRHIS
9   ALAHISARGSERASPALALEUGLNLEUGLY
10   LEUGLYLYSHISASNTYRCYSARGASNPRO
11   ASPASNARGARGARGPROTRPCYSTYRVAL
12   GLNVALGLYLEULYSPROLEUVALGLNGLU
13   CYSMETVALHISASPCYSALAASPGLYLYS

Samples:

sample_one: u-PA amino-terminal fragment, [U-15N], 1.0 mM; D2O 10%; H2O 90%; sodium acetate-d3

sample_two: u-PA amino-terminal fragment, [U-13C; U-15N], 1.0 mM; D2O 10%; H2O 90%; sodium acetate-d3

sample_three: u-PA amino-terminal fragment, [U-13C; U-15N], 2.0 mM; D2O 100%; sodium acetate-d3

sample_conditions_one: pH: 4.5 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker AMX 600 MHz

Related Database Links:

PDB
DBJ BAA00175 BAA01919 BAG35799 BAG60256 BAG60754
EMBL CAA26268 CAA26535 CAA38215 CAH89628
GB AAA61252 AAA61253 AAA73054 AAC97138 AAH13575
PRF 1110198A 1111206A 1111261A 1404232A
REF NP_001124729 NP_001138503 NP_002649 XP_003271314 XP_003312680
SP P00749 Q5RF29