BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4050

Title: 1H, 13C, 15N Chemical Shift Assignments for Reduced Clostridium Pasteurianum Rubredoxin

Authors: Prantner, Andrew; Volkman, Brian; Wilkens, Steven; Xia, Bin; Markley, John

Citation: Prantner, Andrew; Volkman, Brian; Wilkens, Steven; Xia, Bin; Markley, John. "Assignment of 1H, 13C, 15N Signals of Reduced Clostridium Pasteurianum Rubredoxin: Oxidation State-Dependent Changes in Chemical Shifts and Relaxation Rates"  J. Biomol. NMR 10, 411-412 (1997).

Assembly members:
rubredoxin peptide, polymer, 54 residues, 6040 Da.
FE2, non-polymer, 55.845 Da.

Natural source:   Common Name: C. pasteurianum   Taxonomy ID: 1501   Superkingdom: Eubacteria   Kingdom: Monera   Genus/species: Clostridium pasteurianum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
rubredoxin peptide: MKKYTCTVCGYIYNPEDGDP DNGVNPGTDFKDIPDDWVCP LCGVGKDQFEEVEE

Data sets:
Data typeCount
13C chemical shifts150
15N chemical shifts41
1H chemical shifts235

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rubredoxin1
2Fe(II)2

Entities:

Entity 1, rubredoxin 54 residues - 6040 Da.

1   METLYSLYSTYRTHRCYSTHRVALCYSGLY
2   TYRILETYRASNPROGLUASPGLYASPPRO
3   ASPASNGLYVALASNPROGLYTHRASPPHE
4   LYSASPILEPROASPASPTRPVALCYSPRO
5   LEUCYSGLYVALGLYLYSASPGLNPHEGLU
6   GLUVALGLUGLU

Entity 2, Fe(II) - Fe - 55.845 Da.

1   FE2

Samples:

sample_one: Rubredoxin4 – 6 mM; Phosphate buffer mM

sample_conditions_one: pH: 6.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 4051 4066 4137 4182 4319 4320
PDB
GB AAA23279 AJA49845 AJA53833 ELP57804 KER11884
REF WP_003447684
SP P00268