BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4061

Title: Overexpression of Myoglobin and Assignment of Its Amide, C Alpha and C Beta Resonances

Authors: Jennings, Patricia; Stone, Martin; Wright, Peter

Citation: Jennings, Patricia; Stone, Martin; Wright, Peter. "Overexpression of Myoglobin and Assignment of Its Amide, C Alpha and C Beta Resonances"  J. Biomol. NMR 6, 271-276 (1995).

Assembly members:
apomyoglobin, polymer, 154 residues, Formula weight is not available
PROTOPORPHYRIN IX, non-polymer, 562.658 Da.
CARBON MONOXIDE, non-polymer, 28.010 Da.

Natural source:   Common Name: sperm whale   Taxonomy ID: 9755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Physeter catodon

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
apomyoglobin: MVLSEGEWQLVLHVWAKVEA DVAGHGQDILIRLFKSHPET LEKFDRFKHLKTEAEMKASE DLKKHGVTVLTALGAILKKK GHHEAELKPLAQSHATKHKI PIKYLEFISEAIIHVLHSRH PGDFGADAQGAMNKALELFR KDIAAKYKELGYQG

Data sets:
Data typeCount
13C chemical shifts267
15N chemical shifts143
1H chemical shifts143

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apomyoglobin1
2Iron protoporphyrin IX2
3Carbon monoxide3

Entities:

Entity 1, apomyoglobin 154 residues - Formula weight is not available

1   METVALLEUSERGLUGLYGLUTRPGLNLEU
2   VALLEUHISVALTRPALALYSVALGLUALA
3   ASPVALALAGLYHISGLYGLNASPILELEU
4   ILEARGLEUPHELYSSERHISPROGLUTHR
5   LEUGLULYSPHEASPARGPHELYSHISLEU
6   LYSTHRGLUALAGLUMETLYSALASERGLU
7   ASPLEULYSLYSHISGLYVALTHRVALLEU
8   THRALALEUGLYALAILELEULYSLYSLYS
9   GLYHISHISGLUALAGLULEULYSPROLEU
10   ALAGLNSERHISALATHRLYSHISLYSILE
11   PROILELYSTYRLEUGLUPHEILESERGLU
12   ALAILEILEHISVALLEUHISSERARGHIS
13   PROGLYASPPHEGLYALAASPALAGLNGLY
14   ALAMETASNLYSALALEUGLULEUPHEARG
15   LYSASPILEALAALALYSTYRLYSGLULEU
16   GLYTYRGLNGLY

Entity 2, Iron protoporphyrin IX - C34 H34 N4 O4 - 562.658 Da.

1   PP9

Entity 3, Carbon monoxide - C O - 28.010 Da.

1   CMO

Samples:

sample_one: myoglobin carbon monoxide complex, [U-90% 15N; U-95% 13C], 1 mM; potassium phosphate 0.05 M; H2O 90%; D2O 10%

experimental_conditions_MbCO: pH: 5.6; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availableexperimental_conditions_MbCO

Software:

FELIX v2.05, Hare Research -

NMR spectrometers:

  • Bruker AMX500 500 MHz

Related Database Links:

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PDB
DBJ BAF03579
GB AAA72199
PRF 742482A
REF NP_001277651
SP P02185