BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4064

Title: Assignments, Secondary Structure and Dynamics of the Inhibitor-Free Catalytic Fragment of Human Fibroblast Collagenase

Authors: Moy, Franklin; Pisano, Michael; Chanda, Pranab; Urbano, Charlotte; Killar, Loran; Sung, Mei-Li; Powers, Robert

Citation: Moy, Franklin; Pisano, Michael; Chanda, Pranab; Urbano, Charlotte; Killar, Loran; Sung, Mei-Li; Powers, Robert. "Assignments, Secondary Structure and Dynamics of the Inhibitor-Free Catalytic Fragment of Human Fibroblast Collagenase"  J. Biomol. NMR 10, 9-19 (1997).

Assembly members:
human fibroblast collagenase, polymer, 169 residues, 18700 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
human fibroblast collagenase: VLTEGNPRWEQTHLTYRIEN YTPDLPRADVDHAIEKAFQL WSNVTPLTFTKVSEGQADIM ISFVRGDHRDNSPFDGPGGN LAHAFQPGPGIGGDAHFDED ERWTNNFREYNLHRVAAHEL GHSLGLSHSTDIGALMYPSY TFSGDVQLAQDDIDGIQAIY GRSQNPVQP

Data sets:
Data typeCount
13C chemical shifts716
15N chemical shifts158
1H chemical shifts1094

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1collagenase1

Entities:

Entity 1, collagenase 169 residues - 18700 Da.

1   VALLEUTHRGLUGLYASNPROARGTRPGLU
2   GLNTHRHISLEUTHRTYRARGILEGLUASN
3   TYRTHRPROASPLEUPROARGALAASPVAL
4   ASPHISALAILEGLULYSALAPHEGLNLEU
5   TRPSERASNVALTHRPROLEUTHRPHETHR
6   LYSVALSERGLUGLYGLNALAASPILEMET
7   ILESERPHEVALARGGLYASPHISARGASP
8   ASNSERPROPHEASPGLYPROGLYGLYASN
9   LEUALAHISALAPHEGLNPROGLYPROGLY
10   ILEGLYGLYASPALAHISPHEASPGLUASP
11   GLUARGTRPTHRASNASNPHEARGGLUTYR
12   ASNLEUHISARGVALALAALAHISGLULEU
13   GLYHISSERLEUGLYLEUSERHISSERTHR
14   ASPILEGLYALALEUMETTYRPROSERTYR
15   THRPHESERGLYASPVALGLNLEUALAGLN
16   ASPASPILEASPGLYILEGLNALAILETYR
17   GLYARGSERGLNASNPROVALGLNPRO

Samples:

sample_one: human fibroblast collagenase, [U-15N], 1 mM; H2O 90%; D2O 10%; NaCl 100 mM; CaCl2 5 mM; ZnCl2 .1 mM; NaN3 2 mM; deuterated-DTT 10 mM; deuterated-Tris_base 10 mM

sample_two: human fibroblast collagenase, [U-13C;U-15N], 1 mM; D2O 100%; NaCl 100 mM; CaCl2 5 mM; ZnCl2 .1 mM; NaN3 2 mM; deuterated-DTT 10 mM; deuterated-Tris_base 10 mM

sample_conditions: pH: 6.5; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX600 600 MHz

Related Database Links:

PDB
DBJ BAD96754 BAD96755 BAD96756 BAD96776 BAD97001
EMBL CAA28858 CAA38691
GB AAA35699 AAB34887 AAB36941 AAH13875 AAP35520
REF NP_001139410 NP_002412 XP_002822446 XP_003253096 XP_003253097
SP P03956