BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4068

Title: Assignment of 1H, 13C, and 15N Signals of Turkey Ovomucoid Third Domain at pH 2.0

Authors: Choe, Soheui; Dzakula, Zeljko; Kuloglu, E.; Markley, John

Citation: Choe, Soheui; Dzakula, Zeljko; Kuloglu, E.; Markley, John. "Assignment of 1H, 13C, and IN Signals of Turkey Ovomucoid Third Domain at pH 2.0"  J. Biomol. NMR ., .-. (1998).

Assembly members:
turkey ovomucoid third domain, polymer, 56 residues, 6000 Da.

Natural source:   Common Name: turkey   Taxonomy ID: 9103   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Meleagris gallapavo

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
turkey ovomucoid third domain: LAAVSVDCSEYPKPACTLEY RPLCGSDNKTYGNKCNFCNA VVESNGTLTLSHFGKC

Data sets:
Data typeCount
13C chemical shifts191
15N chemical shifts56
1H chemical shifts328

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1OMTKY31

Entities:

Entity 1, OMTKY3 56 residues - 6000 Da.

1   LEUALAALAVALSERVALASPCYSSERGLU
2   TYRPROLYSPROALACYSTHRLEUGLUTYR
3   ARGPROLEUCYSGLYSERASPASNLYSTHR
4   TYRGLYASNLYSCYSASNPHECYSASNALA
5   VALVALGLUSERASNGLYTHRLEUTHRLEU
6   SERHISPHEGLYLYSCYS

Samples:

sample_one: turkey ovomucoid third domain2 – 3 mM; H2O 90%; D2O 10%

sample_two: turkey ovomucoid third domain, [U-15N], 2 – 3 mM; H2O 90%; D2O 10%

sample_three: turkey ovomucoid third domain, [U-15N;13C], 2 – 3 mM; H2O 90%; D2O 10%

sample_conditions: pH: 2.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

FELIX v2.3, Molecular Simulations, Inc - NMR data processing

NMR spectrometers:

  • Bruker DMX-500 500 MHz
  • Bruker DMX-600 600 MHz

Related Database Links:

BMRB 1374 1375 42
PDB
PIR A31445 B61588 C31438 E31437 E31442
SP P05609 P52245 P52263 P67944 P67945