BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4070

Title: Sequence-Specific 1H, 15N and 13C Assignments of the Periplasmic Chaperone FimC from Escherichia coli

Authors: Pellecchia, Maurizio; Guntert, Peter; Glockshuber, Rudi; Wuthrich, Kurt

Citation: Pellecchia, Maurizio; Guntert, Peter; Glockshuber, Rudi; Wuthrich, Kurt. "Sequence-Specific 1H, 15N and 13C Assignments of the 23 kDa Chaperone FimC from Escherichia coli"  J. Biomol. NMR 11, 229-230 (1998).

Assembly members:
chaperone FimC, polymer, 205 residues, 23000 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
chaperone FimC: GVALGATRVIYPAGQKQEQL AVTNNDENSTYLIQSWVENA DGVKDGRFIVTPPLFAMKGK KENTLRILDATNNQLPQDRE SLFWMNVKAIPSMDKSKLTE NTLQLAIISRIKLYYRPAKL ALPPDQAAEKLRFRRSANSL TLINPTPYYLTVTELNAGTR VLENALVPPMGESTVKLPSD AGSNITYRTINDYGALTPKM TGVME

Data sets:
Data typeCount
13C chemical shifts864
15N chemical shifts227
1H chemical shifts1340

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FimC1

Entities:

Entity 1, FimC 205 residues - 23000 Da.

1   GLYVALALALEUGLYALATHRARGVALILE
2   TYRPROALAGLYGLNLYSGLNGLUGLNLEU
3   ALAVALTHRASNASNASPGLUASNSERTHR
4   TYRLEUILEGLNSERTRPVALGLUASNALA
5   ASPGLYVALLYSASPGLYARGPHEILEVAL
6   THRPROPROLEUPHEALAMETLYSGLYLYS
7   LYSGLUASNTHRLEUARGILELEUASPALA
8   THRASNASNGLNLEUPROGLNASPARGGLU
9   SERLEUPHETRPMETASNVALLYSALAILE
10   PROSERMETASPLYSSERLYSLEUTHRGLU
11   ASNTHRLEUGLNLEUALAILEILESERARG
12   ILELYSLEUTYRTYRARGPROALALYSLEU
13   ALALEUPROPROASPGLNALAALAGLULYS
14   LEUARGPHEARGARGSERALAASNSERLEU
15   THRLEUILEASNPROTHRPROTYRTYRLEU
16   THRVALTHRGLULEUASNALAGLYTHRARG
17   VALLEUGLUASNALALEUVALPROPROMET
18   GLYGLUSERTHRVALLYSLEUPROSERASP
19   ALAGLYSERASNILETHRTYRARGTHRILE
20   ASNASPTYRGLYALALEUTHRPROLYSMET
21   THRGLYVALMETGLU

Samples:

sample_one: chaperone FimC, [U-15N],

sample_two: chaperone FimC, [U-13C;U-15N], 1 mM; H20 90%; D2O 10%

sample_conditions_FimC: pH: 5.0; temperature: 311 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX600 600 MHz
  • Bruker DRX500 500 MHz
  • Varian U750+ 750 MHz

Related Database Links:

PDB
DBJ BAB38698 BAE78309 BAG80113 BAI28635 BAI33776
EMBL CAA35967 CAA85729 CAH55780 CAP78797 CAQ34664
GB AAA97212 AAC77272 AAG59498 AAN45626 AAN83817
REF NP_313302 NP_418736 NP_709919 WP_000066501 WP_000066536
SP P31697 P59590