BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4079

Title: Structure of the A-Domain of HMG1 and Its Interaction with DNA As Studied by Heteronuclear Three- and Four-Dimensional NMR Spectroscopy

Authors: Hardman, Colin; Broadhurst, R.; Raine, Andrew; Grasser, Klaus; Thomas, Jean; Laue, Ernest

Citation: Hardman, Colin; Broadhurst, R.; Raine, Andrew; Grasser, Klaus; Thomas, Jean; Laue, Ernest. "Structure of the A-Domain of HMG1 and Its Interaction with DNA As Studied by Heteronuclear Three- and Four-Dimensional NMR Spectroscopy"  Biochemistry 34, 16596-16607 (1995).

Assembly members:
A-domain of HMG1, polymer, 83 residues, Formula weight is not available

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
A-domain of HMG1: GKGDPKKPRGKMSSYAFFVQ TSREEHKKKHPDASVNFSEF SKKCSERWKTMSAKEKGKFE DMAKADKARYEREMKTYIPP KGE

Data sets:
Data typeCount
13C chemical shifts305
15N chemical shifts79
1H chemical shifts581

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1A-domain HMG11

Entities:

Entity 1, A-domain HMG1 83 residues - Formula weight is not available

1   GLYLYSGLYASPPROLYSLYSPROARGGLY
2   LYSMETSERSERTYRALAPHEPHEVALGLN
3   THRSERARGGLUGLUHISLYSLYSLYSHIS
4   PROASPALASERVALASNPHESERGLUPHE
5   SERLYSLYSCYSSERGLUARGTRPLYSTHR
6   METSERALALYSGLULYSGLYLYSPHEGLU
7   ASPMETALALYSALAASPLYSALAARGTYR
8   GLUARGGLUMETLYSTHRTYRILEPROPRO
9   LYSGLYGLU

Samples:

sample_one: A-domain of HMG12 – 5 mM; D2O 10%; H2O 90%; sodium phosphate 10 mM; NaCl 0.15 M; DTT 50 uM; NaN3 .02%

sample_two: A-domain of HMG1, [U-15N], 2 – 5 mM; D2O 10%; H2O 90%; sodium phosphate 10 mM; NaCl 0.15 M; DTT 50 uM; NaN3 .02%

sample_three: A-domain of HMG1, [U-13C], 2 – 5 mM; D2O 10%; H2O 90%; sodium phosphate 10 mM; NaCl 0.15 M; DTT 50 uM; NaN3 .02%

sample_four: A-domain of HMG1, [U-15N;U-13C], 2 – 5 mM; D2O 10%; H2O 90%; sodium phosphate 10 mM; NaCl 0.15 M; DTT 50 uM; NaN3 .02%

sample_conditions: pH: 5.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

BMRB 11147 11532 15148 15149 15502 18709
PDB
DBJ BAA09924 BAC29902 BAC34367 BAC34773 BAC38678
EMBL CAA31110 CAA31284 CAA56631 CAA68441 CAA68526
GB AAA20508 AAA31050 AAA40729 AAA57042 AAA64970
PIR S29857
REF NP_001002937 NP_001004034 NP_001075304 NP_001102843 NP_001162380
SP A9RA84 B0CM99 B1MTB0 P07156 P09429
TPG DAA23902