BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4087

Title: E2 DNA-binding domain from papillomavirus bpv-1

Authors: Veeraraghavan, S.; Mello, C.; Androphy, E.; Baleja, J.

Citation: Veeraraghavan, S.; Mello, C.; Androphy, E.; Baleja, J.. "Structural Correlates for Enhanced Stability in the E2 DNA-Binding Domain from Bovine Papillomavirus"  Biochemistry 38, 16115-16124 (1999).

Assembly members:
E2, polymer, 102 residues, Formula weight is not available

Natural source:   Common Name: BPV-1   Taxonomy ID: 10559   Superkingdom: not available   Kingdom: not available   Genus/species: Bovine papillomavirus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
E2: APRRTTNDGFHLLKAGGSCF ALISGTANQVKCYRFRVKKN HRHRYENCTTTWFTVADNGA ERQGQAQILITFGSPSQRQD FLKHVPLPPGMNISGFTASL DF

Data sets:
Data typeCount
13C chemical shifts336
15N chemical shifts108
1H chemical shifts698

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E21

Entities:

Entity 1, E2 102 residues - Formula weight is not available

1   ALAPROARGARGTHRTHRASNASPGLYPHE
2   HISLEULEULYSALAGLYGLYSERCYSPHE
3   ALALEUILESERGLYTHRALAASNGLNVAL
4   LYSCYSTYRARGPHEARGVALLYSLYSASN
5   HISARGHISARGTYRGLUASNCYSTHRTHR
6   THRTRPPHETHRVALALAASPASNGLYALA
7   GLUARGGLNGLYGLNALAGLNILELEUILE
8   THRPHEGLYSERPROSERGLNARGGLNASP
9   PHELEULYSHISVALPROLEUPROPROGLY
10   METASNILESERGLYPHETHRALASERLEU
11   ASPPHE

Samples:

sample_1: E2, [U-99% 13C], 1.7 mM; Na_posphate 20 mM; Na_chloride 100 mM; dithiothreitol 5 mM; EDTA 0.05 mM; Na_azide 0.01%

sample_2: E2, [U-99% 15N], 1.7 mM; Na_posphate 20 mM; Na_chloride 100 mM; dithiothreitol 5 mM; EDTA 0.05 mM; Na_azide 0.01%

sample_cond_1: pH: 5.75; pressure: 1 atm; temperature: 308.3 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
DBJ BAL04334
GB AFV52369 AGM20697 AGM20699 AGM20701 AGM20703
SP P03122