BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4091

Title: 1H, 15N, 13C, and 13CO Assignments and Secondary Structure Determination of Basic Fibroblast Growth Factor using 3D Heteronuclear NMR Spectroscopy

Authors: Moy, Franklin; Seddon, Andrew; Campbell, Ernest; Bohlen, Peter; Powers, Robert

Citation: Moy, Franklin; Seddon, Andrew; Campbell, Ernest; Bohlen, Peter; Powers, Robert. "1H, 15N, 13C, and 13CO Assignments and Secondary Structure Determination of Basic Fibroblast Growth Factor using 3D Heteronuclear NMR Spectroscopy"  J. Biomol. NMR 6, 245-254 (1995).

Assembly members:
basic fibroblast Growth Factor, polymer, 154 residues, 17200 Da.

Natural source:   Common Name: Human   Taxonomy ID: not available   Superkingdom: eucaryote   Kingdom: Animalia   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
basic fibroblast Growth Factor: AEGEITTLPALPEDGGSGAF PPGHFKDPKRLYCKNGGFFL RIHPDGRVDGVREKSDPHIK LQLQAEERGVVSIKGVSANR YLAMKEDGRLLASKSVTDEC FFFERLESNNYNTYRSRKYT SWYVALKRTGQYKLGSKTGP GQKAILFLPMSAKS

Data sets:
Data typeCount
13C chemical shifts686
15N chemical shifts151
1H chemical shifts1060

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FGF-21

Entities:

Entity 1, FGF-2 154 residues - 17200 Da.

1   ALAGLUGLYGLUILETHRTHRLEUPROALA
2   LEUPROGLUASPGLYGLYSERGLYALAPHE
3   PROPROGLYHISPHELYSASPPROLYSARG
4   LEUTYRCYSLYSASNGLYGLYPHEPHELEU
5   ARGILEHISPROASPGLYARGVALASPGLY
6   VALARGGLULYSSERASPPROHISILELYS
7   LEUGLNLEUGLNALAGLUGLUARGGLYVAL
8   VALSERILELYSGLYVALSERALAASNARG
9   TYRLEUALAMETLYSGLUASPGLYARGLEU
10   LEUALASERLYSSERVALTHRASPGLUCYS
11   PHEPHEPHEGLUARGLEUGLUSERASNASN
12   TYRASNTHRTYRARGSERARGLYSTYRTHR
13   SERTRPTYRVALALALEULYSARGTHRGLY
14   GLNTYRLYSLEUGLYSERLYSTHRGLYPRO
15   GLYGLNLYSALAILELEUPHELEUPROMET
16   SERALALYSSER

Samples:

sample_one: basic fibroblast Growth Factor, [U-15N], 1 mM; DTT 10 mM; H2O 90%; D2O 10%; NaN3 2 mM; potassium phosphate 50 mM

sample_two: basic fibroblast Growth Factor, [U-15N; U-13C], 1 mM; DTT 10 mM; H2O 90%; D2O 10%; NaN3 2 mM; potassium phosphate 50 mM

sample_conditions_one: pH: 5.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AM600 600 MHz

Related Database Links:

BMRB 18995
PDB
DBJ BAG70135 BAG70264
EMBL CCO13911 CCO13913
GB AAA31248 AAA52448 AAA52531 AAA52532 AAA52533
REF NP_001103711 NP_001997 XP_001099284 XP_002815172 XP_003271404
SP P09038 P48799 Q5IS69