BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4092

Title: Complete Heteronuclear NMR Resonance Assignments, Secondary Structure, and Mapping of Core Binding Factor a Binding Site for Core Binding Factor b (1-141)

Authors: Huang, Xuemei; Speck, Nancy; Bushweller, John

Citation: Huang, Xuemei; Speck, Nancy; Bushweller, John. "Complete Heteronuclear NMR Resonance Assignments and Secondary Structure of Core Binding Factor b (1-141)"  J. Biomol. NMR 12, 459-460 (1998).

Assembly members:
Core Binding Factor b subunit, polymer, 143 residues, 16700 Da.

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Core Binding Factor b subunit: GSMPRVVPDQRSKFENEEFF RKLSRECEIKYTGFRDRPHE ERQTRFQNACRDGRSEIAFV ATGTNLSLQFFPASWQGEQR QTPSREYVDLEREAGKVYLK APMILNGVCVIWKGWIDLHR LDGMGCLEFDEERAQQEDAL AQQ

Data sets:
Data typeCount
13C chemical shifts653
15N chemical shifts165
1H chemical shifts1017

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CBF-b(141)1

Entities:

Entity 1, CBF-b(141) 143 residues - 16700 Da.

1   GLYSERMETPROARGVALVALPROASPGLN
2   ARGSERLYSPHEGLUASNGLUGLUPHEPHE
3   ARGLYSLEUSERARGGLUCYSGLUILELYS
4   TYRTHRGLYPHEARGASPARGPROHISGLU
5   GLUARGGLNTHRARGPHEGLNASNALACYS
6   ARGASPGLYARGSERGLUILEALAPHEVAL
7   ALATHRGLYTHRASNLEUSERLEUGLNPHE
8   PHEPROALASERTRPGLNGLYGLUGLNARG
9   GLNTHRPROSERARGGLUTYRVALASPLEU
10   GLUARGGLUALAGLYLYSVALTYRLEULYS
11   ALAPROMETILELEUASNGLYVALCYSVAL
12   ILETRPLYSGLYTRPILEASPLEUHISARG
13   LEUASPGLYMETGLYCYSLEUGLUPHEASP
14   GLUGLUARGALAGLNGLNGLUASPALALEU
15   ALAGLNGLN

Samples:

sample_one: Core Binding Factor b subunit, [U-15N;U-13C], 1.5 mM; potassium phosphate 25 mM; EDTA 0.1 mM; NaN3 0.1%; D2O 5%; H2O 95%

sample_conditions_one: pH: 6.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAA03424 BAA03425 BAA03426 BAC40748 BAE22149
GB AAA02868 AAG01553 AAH06763 AAH18509 AAH26749
REF NP_001013209 NP_001154929 NP_001154930 NP_001178364 NP_001253487
SP Q08024 Q13951
TPG DAA20210 DAA20211