BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4094

Title: 1H, 15N, and 13CO Assignments of Human Interleukin-4 Using Three Dimensional Double- and Triple-Resonance Heteronuclear Magnetic Resonance Spectroscopy

Authors: Powers, Robert; Garrett, Daniel; March, Carl; Frieden, Eric; Gronenborn, Angela; Clore, G.

Citation: Powers, Robert; Garrett, Daniel; March, Carl; Frieden, Eric; Gronenborn, Angela; Clore, G.. "1H, 15N, and 13CO Assignments of Human Interleukin-4 Using Three Dimensional Double- and Triple-Resonance Heteronuclear Magnetic Resonance Spectroscopy"  Biochemistry 31, 4334-4346 (1992).

Assembly members:
human Interleukin 4, polymer, 133 residues, 15400 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Saccharomyces cerevisiae

Entity Sequences (FASTA):
human Interleukin 4: EAEAHKCDITLQEIIKTLNS LTEQKTLCTELTVTDIFAAS KDTTEKETFCRAATVLRQFY SHHEKDTRCLGATAQQFHRH KQLIRFLKRLDRNLWGLAGL NSCPVKEADQSTLENFLERL KTIMREKYSKCSS

Data sets:
Data typeCount
13C chemical shifts605
15N chemical shifts144
1H chemical shifts963

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1IL-41

Entities:

Entity 1, IL-4 133 residues - 15400 Da.

1   GLUALAGLUALAHISLYSCYSASPILETHR
2   LEUGLNGLUILEILELYSTHRLEUASNSER
3   LEUTHRGLUGLNLYSTHRLEUCYSTHRGLU
4   LEUTHRVALTHRASPILEPHEALAALASER
5   LYSASPTHRTHRGLULYSGLUTHRPHECYS
6   ARGALAALATHRVALLEUARGGLNPHETYR
7   SERHISHISGLULYSASPTHRARGCYSLEU
8   GLYALATHRALAGLNGLNPHEHISARGHIS
9   LYSGLNLEUILEARGPHELEULYSARGLEU
10   ASPARGASNLEUTRPGLYLEUALAGLYLEU
11   ASNSERCYSPROVALLYSGLUALAASPGLN
12   SERTHRLEUGLUASNPHELEUGLUARGLEU
13   LYSTHRILEMETARGGLULYSTYRSERLYS
14   CYSSERSER

Samples:

sample_one: human Interleukin 4, [U-95% 15N], 2 mM; H2O 90%; D2O 10%

sample_two: human Interleukin 4, [U-95% 15N; U-95% 13C], 2 mM; D2O 99.996%

sample_conditions: pH: 5.7; temperature: 309 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AM600 600 MHz

Related Database Links:

BMRB 2031
PDB
EMBL CAA34682
GB AAH67515 ABM53124