BMRB Entry 4095

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4095

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

From Genetic to Structural Characterization of a New Class of RNA-Binding Domain Within the SacY/BglG family of Antiterminator

Deposition date:

1998-01-16

Original release date:

1998-09-04

Authors:

Manival, Xavier; Yang, Yinshan; Strub, Marie-paule; Kochoyan, Michel; Steinmetz, Michel; Aymerich, Stephane

Citation:

Citation: Manival, Xavier; Yang, Yinshan; Strub, Marie-paule; Kochoyan, Michel; Steinmetz, Michel; Aymerich, Stephane. "From Genetic to Structural Characterization of a New Class of RNA-Binding Domain Within the SacY/BglG family of Antiterminator" EMBO J. 16, 5019-5029 (1997).

Assembly members:

Assembly members:
sacB mRNA binding domain, polymer, 57 residues, 12992 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
sacB mRNA binding domain: GSMKIKRILNHNAIVVKDQN EEKILLGAGIAFNKKKNDIV DPSKIEKTFIRKDTPDY

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment

Data type	Count
15N chemical shifts	53
1H chemical shifts	423

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Chain A	1
2	Chain B	1

Entities:

Entity 1, Chain A 57 residues - 12992 Da.

1

GLY

SER

MET

LYS

ILE

LYS

ARG

ILE

LEU

ASN

2

HIS

ASN

ALA

ILE

VAL

LYS

ASP

GLN

ASN

3

GLU

LYS

ILE

LEU

GLY

ALA

GLY

ILE

4

ALA

PHE

ASN

LYS

ASN

ASP

ILE

VAL

5

ASP

PRO

SER

LYS

ILE

GLU

LYS

THR

PHE

ILE

6

ARG

LYS

ASP

THR

PRO

ASP

TYR

Samples:

sample_one: sacB mRNA binding domain, [U-15N], 3 mM

sample_conditions: ionic strength: 300 mM; pH: 5.2; temperature: 301 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample_one	not available	sample_conditions

Software:

No software information available

NMR spectrometers:

. . 600 MHz

BMRB	4096
PDB	1AUU
DBJ	BAI87513 BAM55925 BAM59937 GAK81163
EMBL	CAA36720 CAA51569 CAB15868 CCU57104 CEI59651
GB	AAA75336 AEP92902 AFQ59694 AGA22707 AGE65457
REF	NP_391721 WP_003227349 WP_003244589 WP_014478367 WP_015250925
SP	P15401
AlphaFold	P15401