BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4095

Title: From Genetic to Structural Characterization of a New Class of RNA-Binding Domain Within the SacY/BglG family of Antiterminator

Authors: Manival, Xavier; Yang, Yinshan; Strub, Marie-paule; Kochoyan, Michel; Steinmetz, Michel; Aymerich, Stephane

Citation: Manival, Xavier; Yang, Yinshan; Strub, Marie-paule; Kochoyan, Michel; Steinmetz, Michel; Aymerich, Stephane. "From Genetic to Structural Characterization of a New Class of RNA-Binding Domain Within the SacY/BglG family of Antiterminator"  EMBO J. 16, 5019-5029 (1997).

Assembly members:
sacB mRNA binding domain, polymer, 57 residues, 12992 Da.

Natural source:   Common Name: not available   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
sacB mRNA binding domain: GSMKIKRILNHNAIVVKDQN EEKILLGAGIAFNKKKNDIV DPSKIEKTFIRKDTPDY

Data sets:
Data typeCount
15N chemical shifts53
1H chemical shifts423

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Chain A1
2Chain B1

Entities:

Entity 1, Chain A 57 residues - 12992 Da.

1   GLYSERMETLYSILELYSARGILELEUASN
2   HISASNALAILEVALVALLYSASPGLNASN
3   GLUGLULYSILELEULEUGLYALAGLYILE
4   ALAPHEASNLYSLYSLYSASNASPILEVAL
5   ASPPROSERLYSILEGLULYSTHRPHEILE
6   ARGLYSASPTHRPROASPTYR

Samples:

sample_one: sacB mRNA binding domain, [U-15N], 3 mM

sample_conditions: ionic strength: 300 mM; pH: 5.2; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • . . 600 MHz

Related Database Links:

BMRB 4096
PDB
DBJ BAI87513 BAM55925 BAM59937 GAK81163
EMBL CAA36720 CAA51569 CAB15868 CCU57104 CEI59651
GB AAA75336 AEP92902 AFQ59694 AGA22707 AGE65457
REF NP_391721 WP_003227349 WP_003244589 WP_014478367 WP_015250925
SP P15401