BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4097

Title: Backbone 1H, 13C and 15N Chemical Shift Assignment for the HIV-1 RNase H Domain

Authors: Gunther, Kern; Handel, Tracy; Marqusee, Susan

Citation: Gunther, Kern; Handel, Tracy; Marqusee, Susan. "Characterization of a Folding Intermediate from HIV-1 Ribonuclease H"  Protein Sci. 7, 2164-2174 (1998).

Assembly members:
Reverse Transcriptase of HIV-1, polymer, 135 residues, 14885 Da.

Natural source:   Common Name: HIV-1   Taxonomy ID: 11678   Superkingdom: not available   Kingdom: not available   Genus/species: Lentivirus HIV-1

Experimental source:   Production method: chemical construct

Entity Sequences (FASTA):
Reverse Transcriptase of HIV-1: MYQLEKEPIVGAETFYVDGA ANRETKLGKAGYVTNKGRQK VVPLTNTTNQKTELQAIYLA LQDSGLEVNIVTDSQYALGI IQAQPDKSESELVNQIIEQL IKKEKVYLAWVPAHKGIGGN EQVDKLVSAGIRKIL

Data sets:
Data typeCount
13C chemical shifts217
15N chemical shifts111
1H chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNase_H-HIV-1_(RVT)1

Entities:

Entity 1, RNase_H-HIV-1_(RVT) 135 residues - 14885 Da.

1   METTYRGLNLEUGLULYSGLUPROILEVAL
2   GLYALAGLUTHRPHETYRVALASPGLYALA
3   ALAASNARGGLUTHRLYSLEUGLYLYSALA
4   GLYTYRVALTHRASNLYSGLYARGGLNLYS
5   VALVALPROLEUTHRASNTHRTHRASNGLN
6   LYSTHRGLULEUGLNALAILETYRLEUALA
7   LEUGLNASPSERGLYLEUGLUVALASNILE
8   VALTHRASPSERGLNTYRALALEUGLYILE
9   ILEGLNALAGLNPROASPLYSSERGLUSER
10   GLULEUVALASNGLNILEILEGLUGLNLEU
11   ILELYSLYSGLULYSVALTYRLEUALATRP
12   VALPROALAHISLYSGLYILEGLYGLYASN
13   GLUGLNVALASPLYSLEUVALSERALAGLY
14   ILEARGLYSILELEU

Samples:

sample_one: Reverse Transcriptase of HIV-1, [U-99.8% 13C; U-99.9% 15N], 1.1 mM

sample_conditions_one: ionic strength: 0.1; pH: 4.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_onenot availablenot available
HNCAsample_onenot availablenot available
CBCA(CO)NHsample_onenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX600 600 MHz

Related Database Links:

PDB
DBJ BAA12989
GB AAA44198 AAB24839 AAL28068 AAL29457 ADW78894
PRF 1102247C 1103299E
SP P03366 P0C6F2