BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4098

Title: 1H, 15N and 13C Resonance Assignments and Secondary Structure of Apo Liver Fatty Acid-Binding Protein

Authors: Wang, Hsin; He, Yan; Hsu, Kuo Tung; Magliocca, Joseph; Storch, Judith; Stark, Ruth

Citation: Wang, Hsin; He, Yan; Hsu, Kuo Tung; Magliocca, Joseph; Storch, Judith; Stark, Ruth. "1H, 15N and 13C Resonance Assignments and Secondary Structure of Apo Liver Fatty Acid-Binding Protein"  J. Biomol. NMR 12, 197-199 (1998).

Assembly members:
Liver Fatty Acid-Binding Protein, polymer, 127 residues, Formula weight is not available

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Liver Fatty Acid-Binding Protein: MNFSGKYQVQSQENFEPFMK AMGLPEDLIQKGKDIKGVSE IVHEGKKVKLTITYGSKVIH NEFTLGEECELETMTGEKVK AVVKMEGDNKMVTTFKGIKS VTEFNGDTITNTMTLGDIVY KRVSKRI

Data sets:
Data typeCount
13C chemical shifts485
15N chemical shifts109
1H chemical shifts682

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apo-LFABP1

Entities:

Entity 1, apo-LFABP 127 residues - Formula weight is not available

1   METASNPHESERGLYLYSTYRGLNVALGLN
2   SERGLNGLUASNPHEGLUPROPHEMETLYS
3   ALAMETGLYLEUPROGLUASPLEUILEGLN
4   LYSGLYLYSASPILELYSGLYVALSERGLU
5   ILEVALHISGLUGLYLYSLYSVALLYSLEU
6   THRILETHRTYRGLYSERLYSVALILEHIS
7   ASNGLUPHETHRLEUGLYGLUGLUCYSGLU
8   LEUGLUTHRMETTHRGLYGLULYSVALLYS
9   ALAVALVALLYSMETGLUGLYASPASNLYS
10   METVALTHRTHRPHELYSGLYILELYSSER
11   VALTHRGLUPHEASNGLYASPTHRILETHR
12   ASNTHRMETTHRLEUGLYASPILEVALTYR
13   LYSARGVALSERLYSARGILE

Samples:

sample_one: Liver Fatty Acid-Binding Protein, [U-99% 13C; U-98% 15N], 1.3 mM; phosphate 20 mM; D2O 5%; H2O 95%; NaCl 100 mM; EDTA 50 uM; sodium_azide 0.02%; bovine_lung_aprotinin 0.01%

sample_two: Liver Fatty Acid-Binding Protein, [U-98% 15N], 1.0 – 1.3 mM; phosphate 20 mM; D2O 5%; H2O 95%; NaCl 100 mM; EDTA 50 uM; sodium_azide 0.02%; bovine_lung_aprotinin 0.01%

sample_conditions: ionic strength: 0.13 M; pH: 6.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
CBCA(CO)NHnot availablenot availablesample_conditions
HNCACBnot availablenot availablesample_conditions
CBCACOHAnot availablenot availablesample_conditions
H(CCO)NH-TOCSYnot availablenot availablesample_conditions
C(CO)NH-TOCSYnot availablenot availablesample_conditions
HCCH-TOCSYnot availablenot availablesample_conditions
15N-HSQCnot availablenot availablesample_conditions
TOCSY-HSQC(15N)not availablenot availablesample_conditions
NOESY-HSQC(15N)not availablenot availablesample_conditions

Software:

NMRView v3.1 - The majority of assignments were completed by using NMRVIEW

NMRPipe v3.1 - All data were processed with NMRPIPE and then converted to NMRVIEW format for analysis

AutoAssign v3.1 - Preliminary sequential backbone assignments were obtained using AUTOASSIGN program at Rutgers University with CBCA(CO)NH and HNCACB data

PIPP v3.1 -

NMR spectrometers:

  • Unity Inova-600 600 MHz
  • Varian 600 600 MHz

Related Database Links:

BMRB 15429 15433 15434
PDB
EMBL CAA24545
GB AAA41134 AAA41135 AAA41140 AAA42119 AAB19788
PRF 1202232B
REF NP_036688
SP P02692