BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4100

Title: Resonance Assignments of the Mrf-2 DNA-binding Domain

Authors: Yuan, Yate-Ching; Whitson, Robert; Itakura, Keiichi; Chen, Yuan

Citation: Yuan, Yate-Ching; Whitson, Robert; Itakura, Keiichi; Chen, Yuan. "Resonance Assignments of the Mrf-2 DNA-binding Domain"  J. Biomol. NMR 11, 459-460 (1998).

Assembly members:
DNA-binding domain Mrf-2, polymer, 119 residues, 14000 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DNA-binding domain Mrf-2: MRGSHHHHHHGSRADEQAFL VALYKYMKERKTPIERIPYL GFKQINLWTMFQAAQKLGGY ETITARRQWKHIYDELGGNP GSTSAATCTRRHYERLILPY ERFIKGEEDKPLPPIKPRK

Data sets:
Data typeCount
13C chemical shifts349
15N chemical shifts108
1H chemical shifts742

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Mrf-21

Entities:

Entity 1, Mrf-2 119 residues - 14000 Da.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERARGALAASPGLUGLNALAPHELEU
3   VALALALEUTYRLYSTYRMETLYSGLUARG
4   LYSTHRPROILEGLUARGILEPROTYRLEU
5   GLYPHELYSGLNILEASNLEUTRPTHRMET
6   PHEGLNALAALAGLNLYSLEUGLYGLYTYR
7   GLUTHRILETHRALAARGARGGLNTRPLYS
8   HISILETYRASPGLULEUGLYGLYASNPRO
9   GLYSERTHRSERALAALATHRCYSTHRARG
10   ARGHISTYRGLUARGLEUILELEUPROTYR
11   GLUARGPHEILELYSGLYGLUGLUASPLYS
12   PROLEUPROPROILELYSPROARGLYS

Samples:

sample_one: DNA-binding domain Mrf-2, [U-13C; U-15N], 1.0 mM; H2O 90%; D2O 10%; KCL 100 mM; Na2S2O4 20 mM; NaN3 0.15%

sample_conditions: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAB15012 BAE37034 BAI45553
EMBL CAG32224 CAH89369
GB AAA59870 AAF89682 AAH15120 AAH66345 AAI07801
REF NP_001026391 NP_001101094 NP_001125269 NP_001231567 NP_076087
SP E1BLP6 E2R9X2 Q14865 Q5ZJ69 Q8BM75
TPG DAA14307