BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4101

Title: 1H, 13C, and 15N Resonance Assignments of Fusarium Solani Pisi Cutinase and Preliminary Features of the Structure in Solution

Authors: Prompers, Jeanine; Groenewegen, Anneke; van_Schaik, Rene; Pepermans, Henri; Hilbers, Cornelis

Citation: Prompers, Jeanine; Groenewegen, Anneke; van_Schaik, Rene; Pepermans, Henri; Hilbers, Cornelis. "1H, 13C, and 15N Resonance Assignments of Fusarium Solani Pisi Cutinase and Preliminary Features of the Structure in Solution"  Protein Sci. 6, 2374-2384 (1997).

Assembly members:
Fusarium solani pisi cutinase, polymer, 214 residues, 22212 Da.

Natural source:   Common Name: fungus   Taxonomy ID: 29915   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Fusarium solani

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Fusarium solani pisi cutinase: APTSNPAQELEARQLGRTTR DDLINGNSASCADVIFIYAR GSTETGNLGTLGPSIASNLE SAFGKDGVWIQGVGGAYRAT LGDNALPRGTSSAAIREMLG LFQQANTKCPDATLIAGGYS QGAALAAASIEDLDSAIRDK IAGTVLFGYTKNLQNRGRIP NYPADRTKVFCNTGDLVCTG SLIVAAPHLAYGPDARGPAP EFLIEKVRAVRGSA

Data sets:
Data typeCount
13C chemical shifts905
15N chemical shifts230
1H chemical shifts1368

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cutinase1

Entities:

Entity 1, cutinase 214 residues - 22212 Da.

1   ALAPROTHRSERASNPROALAGLNGLULEU
2   GLUALAARGGLNLEUGLYARGTHRTHRARG
3   ASPASPLEUILEASNGLYASNSERALASER
4   CYSALAASPVALILEPHEILETYRALAARG
5   GLYSERTHRGLUTHRGLYASNLEUGLYTHR
6   LEUGLYPROSERILEALASERASNLEUGLU
7   SERALAPHEGLYLYSASPGLYVALTRPILE
8   GLNGLYVALGLYGLYALATYRARGALATHR
9   LEUGLYASPASNALALEUPROARGGLYTHR
10   SERSERALAALAILEARGGLUMETLEUGLY
11   LEUPHEGLNGLNALAASNTHRLYSCYSPRO
12   ASPALATHRLEUILEALAGLYGLYTYRSER
13   GLNGLYALAALALEUALAALAALASERILE
14   GLUASPLEUASPSERALAILEARGASPLYS
15   ILEALAGLYTHRVALLEUPHEGLYTYRTHR
16   LYSASNLEUGLNASNARGGLYARGILEPRO
17   ASNTYRPROALAASPARGTHRLYSVALPHE
18   CYSASNTHRGLYASPLEUVALCYSTHRGLY
19   SERLEUILEVALALAALAPROHISLEUALA
20   TYRGLYPROASPALAARGGLYPROALAPRO
21   GLUPHELEUILEGLULYSVALARGALAVAL
22   ARGGLYSERALA

Samples:

sample_one: Fusarium solani pisi cutinase, [U-99% 13C; U-99% 15N], 2.0 mM; deuterated sodium acetate 10 mM; NaCl 100 mM; sodium azide 0.02%; H2O 95%; D2O 5%

sample_conditions: pH: 5.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

    Related Database Links:

    PDB
    GB AAA33334 AAA33335 AAB05922 EEU36811
    PRF 1008249A
    REF XP_003042524
    SP P00590 Q99174