BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4102

Title: 1H, 13C and 15N NMR Backbone Assignments of 25.5 KDa Metallo-beta-lactamase

Authors: Scrofani, Sergio; Wright, Peter; Dyson, Jane

Citation: Scrofani, Sergio; Wright, Peter; Dyson, Jane. "1H, 13C and 15N NMR Backbone Assignments of 25.5 kDa Metallo-b-lactamase from Bacteroides fragilis"  J. Biomol. NMR 12, 201-202 (1998).

Assembly members:
metallo-beta-lactamase, polymer, 232 residues, 25500 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:   Common Name: B. fragilis   Taxonomy ID: 817   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacteroides fragilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
metallo-beta-lactamase: AQKSVKISDDISITQLSDKV YTYVSLAEIEGWGMVPSNGM IVINNHQAALLDTPINDAQT EMLVNWVTDSLHAKVTTFIP DHWHGDCLGGLGYLQRKGVQ SYANQMTIDLAKEKGLPVPE HGFTDSLTVSLDGMPLQCYY LGGGHATDNIVVWLPTENIL FGGCMLKDNQATSIGNISDA DVTAWPKTLDKVKAKFPSAR YVVPGHGDYGGTELIEHTKQ IVNQYIESTSKP

Data typeCount
13C chemical shifts657
15N chemical shifts213
1H chemical shifts435

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CcrA1
2Zn12
3Zn22

Entities:

Entity 1, CcrA 232 residues - 25500 Da.

1   ALAGLNLYSSERVALLYSILESERASPASP
2   ILESERILETHRGLNLEUSERASPLYSVAL
3   TYRTHRTYRVALSERLEUALAGLUILEGLU
4   GLYTRPGLYMETVALPROSERASNGLYMET
5   ILEVALILEASNASNHISGLNALAALALEU
6   LEUASPTHRPROILEASNASPALAGLNTHR
7   GLUMETLEUVALASNTRPVALTHRASPSER
8   LEUHISALALYSVALTHRTHRPHEILEPRO
9   ASPHISTRPHISGLYASPCYSLEUGLYGLY
10   LEUGLYTYRLEUGLNARGLYSGLYVALGLN
11   SERTYRALAASNGLNMETTHRILEASPLEU
12   ALALYSGLULYSGLYLEUPROVALPROGLU
13   HISGLYPHETHRASPSERLEUTHRVALSER
14   LEUASPGLYMETPROLEUGLNCYSTYRTYR
15   LEUGLYGLYGLYHISALATHRASPASNILE
16   VALVALTRPLEUPROTHRGLUASNILELEU
17   PHEGLYGLYCYSMETLEULYSASPASNGLN
18   ALATHRSERILEGLYASNILESERASPALA
19   ASPVALTHRALATRPPROLYSTHRLEUASP
20   LYSVALLYSALALYSPHEPROSERALAARG
21   TYRVALVALPROGLYHISGLYASPTYRGLY
22   GLYTHRGLULEUILEGLUHISTHRLYSGLN
23   ILEVALASNGLNTYRILEGLUSERTHRSER
24   LYSPRO

Entity 2, Zn1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_one: metallo-beta-lactamase, [U-15N], 0.2 – 1.0 mM; HEPES 10 mM; zinc chloride 10 uM; sodium azide 0.01%; D2O 5%; H2O 95%

sample_two: metallo-beta-lactamase, [U-13C; U-15N], 0.5 – 1.0 mM; HEPES 10 mM; zinc chloride 10 uM; sodium azide 0.01%; D2O 5%; H2O 95%

sample_three: metallo-beta-lactamase, [86%-2H; U-13C; U-15N], 0.8 mM; sodium phosphate 120 mM; D2O 5%; H2O 95%

sample_conditions: pH: 7.0; temperature: 295.0 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX500 500 MHz
  • Bruker DMX600 600 MHz

Related Database Links:

PDB
DBJ BAC06841 BAC06842 BAC06843 BAC06844 BAC06845
EMBL CAH41960 CAQ87572 CAQ87573 CAQ87574 CAQ87575
GB AAA22904 AAA22907 AAM93063 AAR04097 AAR04099
REF WP_005780969 WP_005808062 WP_005821510 WP_015083750 WP_032536010
SP P25910