BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4115

Title: Assignment of 1H, 13C and 15N Signals of the Inhibitor Protein Im9 Bound to the DNase Domain of Colicin E9

Authors: Boetzel, Ruth; Czisch, Michael; MacDonald, Colin; Kaptein, Robert; Hemmings, Andrew; James, Richard; Kleanthous, Colin; Moore, Geoffrey

Citation: Boetzel, Ruth; Czisch, Michael; MacDonald, Colin; Kaptein, Robert; Hemmings, Andrew; James, Richard; Kleanthous, Colin; Moore, Geoffrey. "Assignment of 1H, 13C and 15N Signals of the Inhibitor Protein Im9 Bound to the DNase Domain of Colicin E9"  J. Biomol. NMR 12, 567-568 (1998).

Assembly members:
IM9, polymer, 86 residues, Formula weight is not available
E9, polymer, . residues, Formula weight is not available

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
IM9: MELKHSISDYTEAEFLQLVT TICNADTSSEEELVKLVTHF EEMTEHPSGSDLIYYPKEGD DDSPSGIVNTVKQWRAANGK SGFKQG

Data sets:
Data typeCount
13C chemical shifts254
15N chemical shifts81
1H chemical shifts529

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IM91
2E92

Entities:

Entity 1, IM9 86 residues - Formula weight is not available

1   METGLULEULYSHISSERILESERASPTYR
2   THRGLUALAGLUPHELEUGLNLEUVALTHR
3   THRILECYSASNALAASPTHRSERSERGLU
4   GLUGLULEUVALLYSLEUVALTHRHISPHE
5   GLUGLUMETTHRGLUHISPROSERGLYSER
6   ASPLEUILETYRTYRPROLYSGLUGLYASP
7   ASPASPSERPROSERGLYILEVALASNTHR
8   VALLYSGLNTRPARGALAALAASNGLYLYS
9   SERGLYPHELYSGLNGLY

Entity 2, E9 - Formula weight is not available

Samples:

sample: IM9, [U-100% 15N; U-100% 13C], 1.2 mM; E9, [U-100% 15N; U-100% 13C], 1.3 mM; H2O 90%; D2O 10%

sample_conditions: pH: 6.2 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesamplenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • . . 500 MHz
  • . . 600 MHz

Related Database Links:

BMRB 4116
PDB
EMBL CAA33863
GB AAA23076 ACM07431
REF WP_012644887 YP_002533538
SP P13479