BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4117

Title: 1H, 15N and 13C Chemical Shift Assignment of the Guanine Nucleotide Exchange Domain of Human Elongation Factor-one Beta

Authors: Perez, Janice; Kriek, Jan; Dijk, Jan; Moller, Wim; Siegal, Gregg; Hard, Karl; Kalverda, Arnout; Canters, Gerard

Citation: Perez, Janice; Kriek, Jan; Dijk, Jan; Moller, Wim; Siegal, Gregg; Hard, Karl; Kalverda, Arnout; Canters, Gerard. "1H, 15N and 13C Chemical Shift Assignment of the Guanine Nucleotide Exchange Domain of Human Elongation Factor-one Beta"  J. Biomol. NMR 12, 467-468 (1998).

Assembly members:
human Elongation Factor-1beta, polymer, 91 residues, 10079 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
human Elongation Factor-1beta: MLVAKSSILLDVKPWDDETD MAKLEECVRSIQADGLVWGS SKLVPVGYGIKKLQIQCVVE DDKVGTDMLEEQITAFEDYV QSMDVAAFNKI

Data sets:
Data typeCount
13C chemical shifts370
15N chemical shifts95
1H chemical shifts642

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1hEF-1beta1

Entities:

Entity 1, hEF-1beta 91 residues - 10079 Da.

1   METLEUVALALALYSSERSERILELEULEU
2   ASPVALLYSPROTRPASPASPGLUTHRASP
3   METALALYSLEUGLUGLUCYSVALARGSER
4   ILEGLNALAASPGLYLEUVALTRPGLYSER
5   SERLYSLEUVALPROVALGLYTYRGLYILE
6   LYSLYSLEUGLNILEGLNCYSVALVALGLU
7   ASPASPLYSVALGLYTHRASPMETLEUGLU
8   GLUGLNILETHRALAPHEGLUASPTYRVAL
9   GLNSERMETASPVALALAALAPHEASNLYS
10   ILE

Samples:

sample_one: human Elongation Factor-1beta, [U-13C; U-15N], 1 mM; NaCl 100 mM; NaPO4 10 mM; DTT 1 mM; NaN3 0.02%; H2O 95%; D2O 5%

sample_conditions_one: ionic strength: 0.1 M; pH: 6.9; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAB28447 BAC25661 BAE40151 BAE87819 BAE88158
EMBL CAA43019 CAA43063 CAA52741 CAG32662 CAG33106
GB AAC13264 AAD16874 AAH00211 AAH03899 AAH04931
REF NP_001014936 NP_001032752 NP_001075868 NP_001102269 NP_001230453
SP O70251 P24534 P34826 Q5E983 Q9YGQ1
TPG DAA32476