BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4121

Title: 1H, 13C and 15N Assignment of the Isl-1 Homeodomain

Authors: Ippel, Johannes; Larsson, Goran; Behravan, Gity; Lundqvist, Martin; Lycksell, Per-Olof; Schleucher, Juergen; Zdunek, Janusz; Wijmenga, Sybren

Citation: Ippel, Johannes; Larsson, Goran; Behravan, Gity; Lundqvist, Martin; Lycksell, Per-Olof; Schleucher, Juergen; Zdunek, Janusz; Wijmenga, Sybren. "1H, 13C and 15N Assignment of the Isl-1 Homeodomain"  J. Biomol. NMR 12, 357-359 (1998).

Assembly members:
Isl-1 homeodomain, polymer, 66 residues, 7860 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10117   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus rattus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Isl-1 homeodomain: MKTTRVRTVLNEKQLHTLRT CYAANPRPDALMKEQLVEMT GLSPRVIRVWFQNKRCKDKK RSIMMK

Data sets:
Data typeCount
13C chemical shifts162
15N chemical shifts68
1H chemical shifts474
coupling constants60

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Isl-1-HD1

Entities:

Entity 1, Isl-1-HD 66 residues - 7860 Da.

1   METLYSTHRTHRARGVALARGTHRVALLEU
2   ASNGLULYSGLNLEUHISTHRLEUARGTHR
3   CYSTYRALAALAASNPROARGPROASPALA
4   LEUMETLYSGLUGLNLEUVALGLUMETTHR
5   GLYLEUSERPROARGVALILEARGVALTRP
6   PHEGLNASNLYSARGCYSLYSASPLYSLYS
7   ARGSERILEMETMETLYS

Samples:

sample_one: Isl-1 homeodomain 0.8 mM; DTT 10 mM; NaCl 25 mM; Na2HPO4/NaH2PO4 25 mM; D2O 10%; H2O 90%

sample_two: Isl-1 homeodomain, [U-15N], 0.45 mM; DTT 10 mM; NaCl 25 mM; Na2HPO4/NaH2PO4 25 mM; D2O 10%; H2O 90%

sample_three: Isl-1 homeodomain, [U-15N;U-13C], 1.8 mM; DTT 10 mM; NaCl 25 mM; Na2HPO4/NaH2PO4 25 mM; D2O 10%; H2O 90%

sample_conditions_one: pH: 6.0; temperature: 281 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX600 600 MHz

Related Database Links:

PDB
DBJ BAA04670 BAA07485 BAB29088 BAC41153 BAC57891
EMBL CAA37349 CAB38446 CAI11499 CDQ88523 CDQ89879
GB AAA62161 AAA62171 AAA62172 AAA80274 AAA80275
PIR I67418
PRF 1608207A
REF NP_001092600 NP_001104188 NP_001159513 NP_002193 NP_059035
SP P50211 P50480 P53405 P53407 P53408
TPG DAA17547 DAA17902