BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4123

Title: Main-chain Signal Assignment for the PDZ2 Domain from Human Protein Tyrosine Phosphatase hPTP1E and its Complex with a C-terminal Peptide from the Fas Receptor

Authors: Ekiel, Irena; Banville, Denis; Shen, Shi Hsiang; Slon-Usakiewicz, Jacek; Koshy, Alex; Gehring, Kalle

Citation: Ekiel, Irena; Banville, Denis; Shen, Shi Hsiang; Slon-Usakiewicz, Jacek; Koshy, Alex; Gehring, Kalle. "Main-chain Signal Assignment for the PDZ2Domain from Human Protein Tyrosine Phosphatase hPTP1E and its Complex with a C-terminal Peptide from the Fas Receptor"  J. Biomol. NMR 12, 455-456 (1998).

Assembly members:
second PDZ domain from human phosphatase hPTP1E, polymer, 96 residues, 10006 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
second PDZ domain from human phosphatase hPTP1E: PKPGDIFEVELAKNDNSLGI SVTGGVNTSVRHGGIYVKAV IPQGAAESDGRIHKGDRVLA VNGVSLEGATHKQAVETLRN TGQVVHLLLEKGQSPT

Data sets:
Data typeCount
13C chemical shifts173
15N chemical shifts91
1H chemical shifts329

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ21

Entities:

Entity 1, PDZ2 96 residues - 10006 Da.

1   PROLYSPROGLYASPILEPHEGLUVALGLU
2   LEUALALYSASNASPASNSERLEUGLYILE
3   SERVALTHRGLYGLYVALASNTHRSERVAL
4   ARGHISGLYGLYILETYRVALLYSALAVAL
5   ILEPROGLNGLYALAALAGLUSERASPGLY
6   ARGILEHISLYSGLYASPARGVALLEUALA
7   VALASNGLYVALSERLEUGLUGLYALATHR
8   HISLYSGLNALAVALGLUTHRLEUARGASN
9   THRGLYGLNVALVALHISLEULEULEUGLU
10   LYSGLYGLNSERPROTHR

Samples:

sample_one: second PDZ domain from human phosphatase hPTP1E, [U-15N; U-13C], 3 mM; sodium phosphate buffer 50 mM; sodium chloride 150 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 6.9; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX500 500 MHz

Related Database Links:

BMRB 18833 18834 4124 4516
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