BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4127

Title: Sequence-specific 1H, 13C, and 15N Resonance Assignment of the Origin DNA-binding Domain of SV40 T Antigen

Authors: Luo, Xuelian; Sanford, David; Bullock, Peter; Bachovchin, William

Citation: Luo, Xuelian; Sanford, David; Bullock, Peter; Bachovchin, William. "Solution Structure of the Origin DNA-binding Domain of SV40 T-antigen"  Nat. Struct. Biol. 3, 1034-1039 (1996).

Assembly members:
Origin DNA-binding domain of SV40 T-antigen, polymer, 134 residues, 15390 Da.

Natural source:   Common Name: simian virus 40   Taxonomy ID: 10633   Superkingdom: not available   Kingdom: not available   Genus/species: Polyomavirus Simian virus 40

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Origin DNA-binding domain of SV40 T-antigen: GSKVEDPKDFPSELLSFLSH AVFSNRTLACFAIYTTKEKA ALLYKKIMEKYSVTFISRHN SYNHNILFFLTPHRHRVSAI NNYAQKLCTFSFLICKGVNK EYLMYSALTRDPFSVIEESL PGGLKEHDFNPESS

Data sets:
Data typeCount
13C chemical shifts475
15N chemical shifts130
1H chemical shifts885

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1T-Ag_OBD1

Entities:

Entity 1, T-Ag_OBD 134 residues - 15390 Da.

1   GLYSERLYSVALGLUASPPROLYSASPPHE
2   PROSERGLULEULEUSERPHELEUSERHIS
3   ALAVALPHESERASNARGTHRLEUALACYS
4   PHEALAILETYRTHRTHRLYSGLULYSALA
5   ALALEULEUTYRLYSLYSILEMETGLULYS
6   TYRSERVALTHRPHEILESERARGHISASN
7   SERTYRASNHISASNILELEUPHEPHELEU
8   THRPROHISARGHISARGVALSERALAILE
9   ASNASNTYRALAGLNLYSLEUCYSTHRPHE
10   SERPHELEUILECYSLYSGLYVALASNLYS
11   GLUTYRLEUMETTYRSERALALEUTHRARG
12   ASPPROPHESERVALILEGLUGLUSERLEU
13   PROGLYGLYLEULYSGLUHISASPPHEASN
14   PROGLUSERSER

Samples:

sample_one: Origin DNA-binding domain of SV40 T-antigen, [U-15N;U-13C], 3 mM

sample_conditions_one: pH: 5.5 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
EMBL CAB82893 CBL79143
GB AAB59924 AAC59346 AAD38846 AAD38993 AAD39001
REF YP_003708382
SP P03070