BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4128

Title: NMR Backbone Assignments of the Cyanobacterial Transcriptional Factor, SmtB, that Senses the Zinc Concentration in the Cell

Authors: Morita, Hayato; Kosada, Takashi; Yamazaki, Toshio; Kyogoku, Yoshimasa; Hayashi, Hidenori

Citation: Morita, Hayato; Kosada, Takashi; Yamazaki, Toshio; Kyogoku, Yoshimasa; Hayashi, Hidenori. "NMR Backbone Assignments of the Cyanobacterial Transcriptional Factor, SmtB, that Senses the Zinc Concentration in the Cell"  J. Biomol. NMR 12, 453-454 (1998).

Assembly members:
cyanobacterial metallothionein gene repressor, polymer, 121 residues, 13395.2 Da.

Natural source:   Common Name: Synechococcus   Taxonomy ID: 1140   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Synechococcus PCC7942

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
cyanobacterial metallothionein gene repressor: TKPVLQDGETVVCQGTHAAI ASELQAIAPEVAQSLAEFFA VLADPNRLRLLSLLARSELC VGDLAQAIGVSESAVSHQLR SLRNLRLVSYRKQGRHVYYQ LQDHHIVALYQNALDHLQEC R

Data sets:
Data typeCount
13C chemical shifts119
15N chemical shifts116
1H chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SmtB subunit one1
2SmtB subunit two1

Entities:

Entity 1, SmtB subunit one 121 residues - 13395.2 Da.

1   THRLYSPROVALLEUGLNASPGLYGLUTHR
2   VALVALCYSGLNGLYTHRHISALAALAILE
3   ALASERGLULEUGLNALAILEALAPROGLU
4   VALALAGLNSERLEUALAGLUPHEPHEALA
5   VALLEUALAASPPROASNARGLEUARGLEU
6   LEUSERLEULEUALAARGSERGLULEUCYS
7   VALGLYASPLEUALAGLNALAILEGLYVAL
8   SERGLUSERALAVALSERHISGLNLEUARG
9   SERLEUARGASNLEUARGLEUVALSERTYR
10   ARGLYSGLNGLYARGHISVALTYRTYRGLN
11   LEUGLNASPHISHISILEVALALALEUTYR
12   GLNASNALALEUASPHISLEUGLNGLUCYS
13   ARG

Samples:

sample_one: cyanobacterial metallothionein gene repressor, [U-15N];[U-13C,U-15N];[15N]-His;[15N]-Lys;[15N]-Arg;[15N]-Val;[15N]-Leu;[15N]-Cys, 0.6 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 6.0; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D_1H-15N_HSQCsample_onenot availablesample_conditions_one
Three-dimensional experimentssample_onenot availablesample_conditions_one
3D_HNCACBsample_onenot availablesample_conditions_one
3D_CBCA(CO)NHsample_onenot availablesample_conditions_one
3D_HNCAsample_onenot availablesample_conditions_one
3D_HN(CO)CAsample_onenot availablesample_conditions_one

Software:

NMRPipe v1.6, NIH - peak picking and assignments

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 4306
PDB
DBJ BAD78452
EMBL CAA45872
GB AAT01609 AAT01610 AAT01611 AAT01612 AAT01613
REF WP_011242576
SP P30340