BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4149

Title: Solution Structure of Gallium-Substituted Putidaredoxin Mutant: GaPdx C85S

Authors: Pochapsky, Thomas; Kuti, Miklos; Kazanis, Sophia

Citation: Pochapsky, Thomas; Kuti, Miklos; Kazanis, Sophia. "Solution Structure of Gallium-Substituted Putidaredoxin Mutant: GaPdx C85S"  J. Biomol. NMR 12, 407-415 (1998).

Assembly members:
putidaredoxin, polymer, 106 residues, Formula weight is not available
GALLIUM (III) ION, non-polymer, 69.723 Da.

Natural source:   Common Name: Pseudomonas putida   Taxonomy ID: 303   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas putida

Experimental source:   Production method: recombinant_technology

Entity Sequences (FASTA):
putidaredoxin: SKVVYVSHDGTRRELDVADG VSLMQAAVSNGIYDIVGDCG GSASCATCHVYVNEAFTDKV PAANEREIGMLECVTAELKP NSRLSCQIIMTPELDGIVVD VPDRQW

Data sets:
Data typeCount
13C chemical shifts446
15N chemical shifts114
1H chemical shifts720

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pdx-protein1
2entity_GA2

Entities:

Entity 1, Pdx-protein 106 residues - Formula weight is not available

1   SERLYSVALVALTYRVALSERHISASPGLY
2   THRARGARGGLULEUASPVALALAASPGLY
3   VALSERLEUMETGLNALAALAVALSERASN
4   GLYILETYRASPILEVALGLYASPCYSGLY
5   GLYSERALASERCYSALATHRCYSHISVAL
6   TYRVALASNGLUALAPHETHRASPLYSVAL
7   PROALAALAASNGLUARGGLUILEGLYMET
8   LEUGLUCYSVALTHRALAGLULEULYSPRO
9   ASNSERARGLEUSERCYSGLNILEILEMET
10   THRPROGLULEUASPGLYILEVALVALASP
11   VALPROASPARGGLNTRP

Entity 2, entity_GA - Ga - 69.723 Da.

1   GA

Samples:

sample_one: gallium putidaredoxin 2 mM; Tris buffer 0.01 M; mercapatoethanol trace

sample_conditions_one: pH: 7.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_onenot availablesample_conditions_one
Flipback NOESYsample_onenot availablesample_conditions_one
15N-edited NOESY,TOCSY (3D)sample_onenot availablesample_conditions_one
HNCAsample_onenot availablesample_conditions_one
HN(CO)CAsample_onenot availablesample_conditions_one
HCCH-TOCSYsample_onenot availablesample_conditions_one
13C-edited NOESY (3D)sample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

    Related Database Links:

    BMRB 1551 2278 4154
    PDB
    DBJ BAA00414 BAN13288
    GB AAA25759
    REF WP_032492635 YP_009083114
    SP P00259