BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4165

Title: 1H, 13C, and 15N Chemical Shift Assignments for Tn916 N-terminal DNA Binding Domain complex with DNA   PubMed: 10382314

Authors: Wojciak, Jonathan; Connolly, Kevin; Clubb, Robert

Citation: Connolly, Kevin; Wojciak, Jonathan; Clubb, Robert. "Resonance assignments of the Tn916 integrase DNA-binding domain and the integrase:DNA complex"  J. Biomol. NMR 14, 95-96 (1999).

Assembly members:
Tn916 integrase, polymer, 74 residues, Formula weight is not available
DNA binding site of Tn916 integrase, polymer, 13 residues, Formula weight is not available
DNA binding site of Tn916 integrase, polymer, 13 residues, Formula weight is not available

Natural source:   Common Name: Enterococcus faecalis   Taxonomy ID: 1351   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Enterococcus faecalis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Tn916 integrase: MSEKRRDNRGRILKTGESQR KDGRYLYKYIDSFGEPQFVY SWKLVATDRVPAGKRDAISL REKIAELQKDIHDG
DNA binding site of Tn916 integrase: GAGTAGTAAATTC
DNA binding site of Tn916 integrase: GAATTTACTACTC

Data typeCount
13C chemical shifts231
15N chemical shifts80
1H chemical shifts667

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tn916int1
2DNA 12
3DNA 23

Entities:

Entity 1, Tn916int 74 residues - Formula weight is not available

1   METSERGLULYSARGARGASPASNARGGLY
2   ARGILELEULYSTHRGLYGLUSERGLNARG
3   LYSASPGLYARGTYRLEUTYRLYSTYRILE
4   ASPSERPHEGLYGLUPROGLNPHEVALTYR
5   SERTRPLYSLEUVALALATHRASPARGVAL
6   PROALAGLYLYSARGASPALAILESERLEU
7   ARGGLULYSILEALAGLULEUGLNLYSASP
8   ILEHISASPGLY

Entity 2, DNA 1 13 residues - Formula weight is not available

1   DGDADGDTDADGDTDADADA
2   DTDTDC

Entity 3, DNA 2 13 residues - Formula weight is not available

1   DGDADADTDTDTDADCDTDA
2   DCDTDC

Samples:

sample_one: Tn916 integrase 1.0 mM; DNA binding site of Tn916 integrase 1.0 mM; DNA binding site of Tn916 integrase 1.0 mM

sample_conditions_one: pH: 6.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

PIPP - peak assignment

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

BMRB 4160
PDB
DBJ BAG12510 BAK28444 BAN28951 GAD37680
EMBL CAA43360 CAO82956 CAQ49380 CAV31149 CAZ51601
GB AAA19427 AAB60030 AAC36982 AAM99801 AAY63950
PRF 2114402Y
REF NP_687929 WP_001291559 WP_001291560 WP_001291561 WP_001291562
SP P22886 P62904 P62905