BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4193

Title: Sequence-Specific 1H, 15N and 13C Assignment of Adenylate Kinase from Escherichia coli in Complex with the Inhibitor AP5A

Authors: Meirovitch, Eva; Sinev, Michael; Sineva, Elena

Citation: Meirovitch, Eva; Sinev, Michael; Sineva, Elena. "Letter to the Editor: Sequence-Specific 1H, 15N and 13C Assignment of Adenylate Kinase from Escherichia coli in Complex with the Inhibitor AP5A"  J. Biomol. NMR 13, 195-196 (1999).

Assembly members:
Adenylate kinase, polymer, 214 residues, Formula weight is not available
AP5, non-polymer, 916.367 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Adenylate kinase: MRIILLGAPGAGKGTQAQFI MEKYGIPQISTGDMLRAAVK SGSELGKQAKDIMDAGKLVT DELVIALVKERIAQEDCRNG FLLDGFPRTIPQADAMKEAG INVDYVLEFDVPDELIVDRI VGRRVHAPSGRVYHVKFNPP KVEGKDDVTGEELTTRKDDQ EETVRKRLVEYHQMTAPLIG YYSKEAEAGNTKYAKVDGTK PVAEVRADLEKILG

Data sets:
Data typeCount
13C chemical shifts859
15N chemical shifts220
1H chemical shifts1424

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Akeco1
2AP52

Entities:

Entity 1, Akeco 214 residues - Formula weight is not available

1   METARGILEILELEULEUGLYALAPROGLY
2   ALAGLYLYSGLYTHRGLNALAGLNPHEILE
3   METGLULYSTYRGLYILEPROGLNILESER
4   THRGLYASPMETLEUARGALAALAVALLYS
5   SERGLYSERGLULEUGLYLYSGLNALALYS
6   ASPILEMETASPALAGLYLYSLEUVALTHR
7   ASPGLULEUVALILEALALEUVALLYSGLU
8   ARGILEALAGLNGLUASPCYSARGASNGLY
9   PHELEULEUASPGLYPHEPROARGTHRILE
10   PROGLNALAASPALAMETLYSGLUALAGLY
11   ILEASNVALASPTYRVALLEUGLUPHEASP
12   VALPROASPGLULEUILEVALASPARGILE
13   VALGLYARGARGVALHISALAPROSERGLY
14   ARGVALTYRHISVALLYSPHEASNPROPRO
15   LYSVALGLUGLYLYSASPASPVALTHRGLY
16   GLUGLULEUTHRTHRARGLYSASPASPGLN
17   GLUGLUTHRVALARGLYSARGLEUVALGLU
18   TYRHISGLNMETTHRALAPROLEUILEGLY
19   TYRTYRSERLYSGLUALAGLUALAGLYASN
20   THRLYSTYRALALYSVALASPGLYTHRLYS
21   PROVALALAGLUVALARGALAASPLEUGLU
22   LYSILELEUGLY

Entity 2, AP5 - C20 H29 N10 O22 P5 - 916.367 Da.

1   AP5

Samples:

sample_one: Adenylate kinase/AP5A complex, [U-15N], 2.7 – 5.7 mM; sodium-phosphate buffer 40 mM; sodium azide 1 uM; H2O 95%; D2O 5%

sample_two: Adenylate kinase/AP5A complex, [U-15N; U-13C], 2.7 – 5.7 mM; sodium-phosphate buffer 40 mM; sodium azide 1 uM; H2O 95%; D2O 5%

sample_conditions_one: pH: 6.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCACBsample_onenot availablesample_conditions_one
CCONHsample_onenot availablesample_conditions_one
HCCONHsample_onenot availablesample_conditions_one
15N HSQCsample_onenot availablesample_conditions_one
13C,HSQCsample_onenot availablesample_conditions_one
HCCH_TOCSYsample_onenot availablesample_conditions_one
HCCH_COSYsample_onenot availablesample_conditions_one
3D N_NOESYsample_onenot availablesample_conditions_one
3D C_NOESYsample_onenot availablesample_conditions_one
3D CBCA(CO)NHsample_onenot availablesample_conditions_one
3D HBHA(CBCACO)NHsample_onenot availablesample_conditions_one
HNCOsample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 18683 18685 18686 18687 19089 19090 19091 19092 19093 25353 25357 25360 25361 25362 4152 4350
PDB
DBJ BAA14303 BAB33950 BAE76253 BAG76023 BAI23848
EMBL CAA26840 CAF33430 CAF33431 CAF33432 CAF33433
GB AAA23461 AAB40228 AAC73576 AAG54823 AAM94352
REF NP_308554 NP_415007 NP_706367 WP_001220233 WP_001220235
SP A7ZIN4 A7ZXD2 B1IZC0 B1LJN2 B1XFR1