BMRB Entry 4197

Title:
Solution NMR Structures of the Major Coat Protein of Filamentous Bacteriophage M13 Solubilized in Sodium Docecyl Sulphate Micelles, 25 Lowest Energy Structures
Deposition date:
1998-09-09
Original release date:
1999-11-24
Authors:
Papavoine, C.; Christiaans, B.; Folmer, R.; Konings, R.; Hilbers, C.
Citation:

Citation: Papavoine, C.; Christiaans, B.; Folmer, R.; Konings, R.; Hilbers, C.. "Solution Structure of the M13 Major Coat Protein in Detergent Micelles: A Basis for a Model of Phage Assembly Involving Specific Residues."  J. Mol. Biol. 282, 401-419 (1998).

Assembly members:

Assembly members:
M13 major coat protein, polymer, 50 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: bacteriophage_M13   Taxonomy ID: 10870   Superkingdom: not available   Kingdom: not available   Genus/species: Inovirus bacteriophage_M13

Experimental source:

Experimental source:   Production method: natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
M13 major coat protein: AEGDDPAKAAFNSLQASATE YIGYAWAMVVVIVGATIGIK LFKKFTSKAS

Data sets:
Data typeCount
13C chemical shifts204
15N chemical shifts51
1H chemical shifts316

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gVIIIp1

Entities:

Entity 1, gVIIIp 50 residues - Formula weight is not available

1   ALAGLUGLYASPASPPROALALYSALAALA
2   PHEASNSERLEUGLNALASERALATHRGLU
3   TYRILEGLYTYRALATRPALAMETVALVAL
4   VALILEVALGLYALATHRILEGLYILELYS
5   LEUPHELYSLYSPHETHRSERLYSALASER

Samples:

sample_one: M13 major coat protein, [U-100% 13C; U-100% 15N], 1.5 – 2 mM

sample_conditions_one: pH: 4.9; temperature: 311 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_onenot availablenot available
TOCSYsample_onenot availablenot available
NOESYsample_onenot availablenot available
NOESY-HMQC (15N and 13C)sample_onenot availablenot available
TOCSY-HMQC (15N)sample_onenot availablenot available
HCCH-TOCSYsample_onenot availablenot available
ROESY-GHSQCsample_onenot availablenot available
HMQC-NOESY-GHSQCsample_onenot availablenot available
HMQC-J,HNHAsample_onenot availablenot available
HACACB-COSYsample_onenot availablenot available
HNHB,HSQC(3J CGN)sample_onenot availablenot available
HSQC(3JCGC')sample_onenot availablenot available

Software:

MNMR - FT of the recorded spectra

XEASY v3.9.1 - Analysis of the spectra to obtain assignments and structural information

NMR spectrometers:

  • Varian Unity+ 400 MHz
  • Bruker AM 500 MHz
  • Bruker AMX 600 MHz

Related Database Links:

BMRB 17728 19734 19747 2590 2591 2592 4209
PDB
EMBL CAA23861 CAA23871
GB AAA32207 AAA32214 AAA32220 AAA32308 AAB24445
PRF 0812197K
REF NP_510890 YP_009111292 YP_009111302
SP P69539 P69540 P69541
AlphaFold P69539 P69540 P69541

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks