BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4256

Title: 1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from S. cerevisiae in Both Its Free and DNA Bound Forms, and 1H Assignments of the Free DNA

Authors: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew

Citation: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew. "1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from S. cerevisiae in Both Its Free and DNA Bound Forms, and 1H Assignments of the Free DNA"  J. Biomol. NMR 13, 397-398 (1999).

Assembly members:
MLU1-BOX binding protein, polymer, 135 residues, Formula weight is not available
DNA binding domain of Mbp1, polymer, 13 residues, Formula weight is not available

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota fungi

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
MLU1-BOX binding protein: SNQIYSARYSGVDVYEFIHS TGSIMKRKKDDWVNATHILK AANFAKAKRTRILEKEVLKE THEKVQGGFGKYQGTWVPLN IAKQLAEKFSVYDQLKPLFD FTQTDGSASPPPAPKHHHAS KVDKLAAALEHHHHH
DNA binding domain of Mbp1: TTTGACGCGTCAA

Data sets:
Data typeCount
13C chemical shifts156
15N chemical shifts115
1H chemical shifts419

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mbp1(2-124)1
2DNA strand 12
3DNA strand 22

Entities:

Entity 1, Mbp1(2-124) 135 residues - Formula weight is not available

1   SERASNGLNILETYRSERALAARGTYRSER
2   GLYVALASPVALTYRGLUPHEILEHISSER
3   THRGLYSERILEMETLYSARGLYSLYSASP
4   ASPTRPVALASNALATHRHISILELEULYS
5   ALAALAASNPHEALALYSALALYSARGTHR
6   ARGILELEUGLULYSGLUVALLEULYSGLU
7   THRHISGLULYSVALGLNGLYGLYPHEGLY
8   LYSTYRGLNGLYTHRTRPVALPROLEUASN
9   ILEALALYSGLNLEUALAGLULYSPHESER
10   VALTYRASPGLNLEULYSPROLEUPHEASP
11   PHETHRGLNTHRASPGLYSERALASERPRO
12   PROPROALAPROLYSHISHISHISALASER
13   LYSVALASPLYSLEUALAALAALALEUGLU
14   HISHISHISHISHIS

Entity 2, DNA strand 1 13 residues - Formula weight is not available

1   DTDTDTDGDADCDGDCDGDT
2   DCDADA

Samples:

sample_one: MLU1-BOX binding protein, [U-15N; U-13C], 0.8 mM; DNA binding domain of Mbp11.0 – 1.68 mM; Na phosphate 40 mM; NaCl 110 mM; D2O 10%; H2O 90%

sample_two: MLU1-BOX binding protein 0.5 mM; DNA binding domain of Mbp11.0 – 1.68 mM; Na phosphate 40 mM; NaCl 110 mM; D2O 10%; H2O 90%

sample_conditions_one: pH: 7.6; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

    Related Database Links:

    BMRB 4254
    PDB
    DBJ GAA22190
    EMBL CAA52271 CAA98618 CAY78452
    GB AAS64332 AAS64333 AAS64335 AAS64336 AAS64338
    REF NP_010227
    SP P39678
    TPG DAA11800