BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4259

Title: Sequential Assignment and Solution Secondary Structure of Doubly Labelled Ribonuclease Sa

Authors: Laurents, Douglas; Perez-Canadillas, Jose Manuel; Bruix, Marta; Santoro, Jorge; Schell, David; Hebert, Eric; Pace, C.; Rico, Manuel

Citation: Laurents, Douglas; Perez-Canadillas, Jose Manuel; Santoro, Jorge; Rico, Manuel; Schell, David; Hebert, Eric; Pace, C.; Bruix, Marta. "Sequential Assignment and Solution Secondary Structure of Doubly Labelled Ribonuclease Sa"  J. Biomol. NMR 14, 89-90 (1999).

Assembly members:
RNAse-SA, polymer, 96 residues, Formula weight is not available

Natural source:   Common Name: S. aureofaciens   Taxonomy ID: 1894   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptomyces aureofaciens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RNAse-SA: DVSGTVCLSALPPEATDTLN LIASDGPFPYSQDGVVFQNR ESVLPTQSYGYYHEYTVITP GARTRGTRRIITGEATQEDY YTGDHYATFSLIDQTC

Data sets:
Data typeCount
13C chemical shifts183
15N chemical shifts96
1H chemical shifts635

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNAse-SA1

Entities:

Entity 1, RNAse-SA 96 residues - Formula weight is not available

1   ASPVALSERGLYTHRVALCYSLEUSERALA
2   LEUPROPROGLUALATHRASPTHRLEUASN
3   LEUILEALASERASPGLYPROPHEPROTYR
4   SERGLNASPGLYVALVALPHEGLNASNARG
5   GLUSERVALLEUPROTHRGLNSERTYRGLY
6   TYRTYRHISGLUTYRTHRVALILETHRPRO
7   GLYALAARGTHRARGGLYTHRARGARGILE
8   ILETHRGLYGLUALATHRGLNGLUASPTYR
9   TYRTHRGLYASPHISTYRALATHRPHESER
10   LEUILEASPGLNTHRCYS

Samples:

sample_one: RNAse-SA1.5 – 2.0 mM; H2O 90%; D2O 10%

sample_two: RNAse-SA1.5 – 2.0 mM; D2O 100%

sample_three: RNAse-SA, [U-15N], 1.5 – 2.0 mM; H2O 90%; D2O 10%

sample_four: RNAse-SA, [U-15N; 13C], 1.5 – 2.0 mM; H2O 90%; D2O 10%

sample_conditions_one: pH*: 5.5; temperature: 303.2 K

sample_conditions_two: pH*: 5.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
COSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

ANSIG v3.3 - Used for assigning the spectra. Automatic peak picking in 2D

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

PDB
GB AAA81522 KND14426
REF WP_030287817 WP_046385930
SP P05798