BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4270

Title: Chemical Shift Assignment of Ca2+/calmodulin (1H, 13C, and 15N) Complexed with Its Binding Domain from Rat Ca2+/calmodulin Dependent Protein Kinase Kinase (1H).

Authors: Osawa, Masanori; Tokumitsu, Hiroshi; Swindells, Mark; Kurihara, Hiroyuki; Orita, Masaya; Shibanuma, Tadao; Furuya, Toshio; Ikura, Mitsuhiko

Citation: Osawa, Masanori; Tokumitsu, Hiroshi; Swindells, Mark; Kurihara, Hiroyuki; Orita, Masaya; Shibanuma, Tadao; Furuya, Toshio; Ikura, Mitsuhiko. "A Novel Target Recognition Revealed by Calmodulin in Complex with Ca2+-calmodulin-dependent Kinase Kinase"  Nat. Struct. Biol. 6, 819-824 (1999).

Assembly members:
calmodulin, polymer, 148 residues, 40.078 Da.
Ca2+/calmodulin dependent protein kinase kinase complex, polymer, 26 residues, 40.078 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
Ca2+/calmodulin dependent protein kinase kinase complex: VKLIPSWTTVILVKSMLRKR SFGNPF

Data sets:
Data typeCount
13C chemical shifts601
15N chemical shifts153
1H chemical shifts1137

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1
2camkk2

Entities:

Entity 1, calmodulin 148 residues - 40.078 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, camkk 26 residues - 40.078 Da.

1   VALLYSLEUILEPROSERTRPTHRTHRVAL
2   ILELEUVALLYSSERMETLEUARGLYSARG
3   SERPHEGLYASNPROPHE

Samples:

sample_one: calmodulin, [U-13C, U-15N], 1.5 mM; Ca2+/calmodulin dependent protein kinase kinase complex 1.875 mM; potassium chloride 100 mM; calcium chloride 10.6 mM

sample_conditions_one: pH: 6.7; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4284 4310
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863
PIR JC1305 MCON
PRF 0409298A 0608335A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234
SP O02367 O16305 O96081 P02594 P05932
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988