BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4280

Title: Sequence Specific 1H, 13C and 15N Assignment of the Methyl Binding Domain of the Methyl-CpG-binding Protein MeCP2.

Authors: Wakefield, Robert; Smith, Brian; Nan, Xinsheng; Free, Andrew; Soteriou, Alice; Uhrin, Dusan; Bird, Adrian; Barlow, Paul

Citation: Wakefield, Robert; Smith, Brian; Nan, Xinsheng; Free, Andrew; Soteriou, Alice; Uhrin, Dusan; Bird, Adrian; Barlow, Paul. "The solution structure of the domain from MeCP2 that binds to methylated DNA"  J. Mol. Biol. 291, 1055-1065 (1999).

Assembly members:
Methyl binding domain of MeCP2, polymer, 92 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eucaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Methyl binding domain of MeCP2: ASASPKQRRSIIRDRGPMYD DPTLPEGWTRKLKQRKSGRS AGKYDVYLINPQGKAFRSKV ELIAYFEKVGDTSLDPNDFD FTVTGRGSGSGC

Data sets:
Data typeCount
13C chemical shifts291
15N chemical shifts91
1H chemical shifts618

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MBD1

Entities:

Entity 1, MBD 92 residues - Formula weight is not available

1   ALASERALASERPROLYSGLNARGARGSER
2   ILEILEARGASPARGGLYPROMETTYRASP
3   ASPPROTHRLEUPROGLUGLYTRPTHRARG
4   LYSLEULYSGLNARGLYSSERGLYARGSER
5   ALAGLYLYSTYRASPVALTYRLEUILEASN
6   PROGLNGLYLYSALAPHEARGSERLYSVAL
7   GLULEUILEALATYRPHEGLULYSVALGLY
8   ASPTHRSERLEUASPPROASNASPPHEASP
9   PHETHRVALTHRGLYARGGLYSERGLYSER
10   GLYCYS

Samples:

sample_one: Methyl binding domain of MeCP2, [U-15N; U-13C], 1.0 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 6.0; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 4467
PDB
DBJ BAE34602 BAF82133 BAG73396
EMBL CAA61599 CAA64331 CAA68001 CAA73190 CAA74577
GB AAA41584 AAC08757 AAC32737 AAC68880 AAF33023
PIR S57963
REF NP_001075448 NP_001104262 NP_001164841 NP_001193481 NP_001244471
SP P51608 Q00566 Q95LG8 Q9Z2D6