BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4284

Title: NMR Solution Structure of a Complex of Calmodulin with a Binding Peptide of the Ca2+-Pump

Authors: Elshorst, Bettina; Hennig, Mirko; Foersterling, Holger; Diener, Alexander; Maurer, Marcus; Schulte, Petra; Schwalbe, Harald; Griesinger, Christian; Krebs, Joachim; Schmid, Holger; Vorherr, Thomas; Carafoli, Ernesto

Citation: Elshorst, Bettina; Hennig, Mirko; Foersterling, Holger; Diener, Alexander; Maurer, Marcus; Schulte, Petra; Schwalbe, Harald; Griesinger, Christian; Krebs, Joachim; Schmid, Holger; Vorherr, Thomas; Carafoli, Ernesto. "NMR Solution Structure of a Complex of Calmodulin with a Binding Peptide of the Ca2+-Pump"  Biochemistry 38, 12320-12332 (1999).

Assembly members:
Calmodulin, polymer, 148 residues, Formula weight is not available
C20W peptide, polymer, 20 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
C20W peptide: LRRGQILWFRGLNRIQTQIK

Data typeCount
13C chemical shifts556
15N chemical shifts143
1H chemical shifts987

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cam1
2C20W2
3Ca3

Entities:

Entity 1, cam 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, C20W 20 residues - Formula weight is not available

1   LEUARGARGGLYGLNILELEUTRPPHEARG
2   GLYLEUASNARGILEGLNTHRGLNILELYS

Entity 3, Ca - Ca - 40.078 Da.

1   CA

Samples:

sample_one: Calmodulin 1.7 mM; C20W peptide 1.7 mM; KCl 100 mM; CaCl2 5 mM

sample_conditions_one: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCsample_onenot availablesample_conditions_one
13C-HSQCsample_onenot availablesample_conditions_one
HNCOsample_onenot availablesample_conditions_one
HNCAsample_onenot availablesample_conditions_one
CBCACONHsample_onenot availablesample_conditions_one
CBCANHsample_onenot availablesample_conditions_one
HBHA(CO)NHsample_onenot availablesample_conditions_one
HCCH-TOCSYsample_onenot availablesample_conditions_one
(H)CC(CO)NHsample_onenot availablesample_conditions_one
H(CCCO)NHsample_onenot availablesample_conditions_one
NOESY-13C-HSQCsample_onenot availablesample_conditions_one
NOESY-15N-HSQCsample_onenot availablesample_conditions_one
(HB)CB(CCarom)Hsample_onenot availablesample_conditions_one
HMBC-HSQCsample_onenot availablesample_conditions_one
LRCCsample_onenot availablesample_conditions_one
12C,14N-NOESYsample_onenot availablesample_conditions_one

Software:

FELIX v970, Molecular Simulations Inc. - resonance assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4270 4310
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863
PIR JC1305 MCON
PRF 0409298A 0608335A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234
SP O02367 O16305 O96081 P02594 P05932
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988