BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4293

Title: Assignment of 1H, 13C and 15N Signals of the DNase Domain of Colicin E9

Authors: Whittaker, Sara; Boetzel, Ruth; MacDonald, Colin; Lian, Lu-Yun; James, Richard; Kleanthous, Colin; Moore, Geoffrey

Citation: Whittaker, Sara; Boetzel, Ruth; MacDonald, Colin; Lian, Lu-Yun; James, Richard; Kleanthous, Colin; Moore, Geoffrey. "Assignment of 1H, 13C and 15N Signals of the DNase Domain of Colicin E9"  J. Biomol. NMR ., .-..

Assembly members:
DNase domain of colicin E9, polymer, 134 residues, 15090 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DNase domain of colicin E9: MESKRNKPGKATGKGKPVGD KWLDDAGKDSGAPIPDRIAD KLRDKEFKSFDDFRKAVWEE VSKDPELSKNLNPSNKSSVS KGYSPFTPKNQQVGGRKVYE LHHDKPISQGGEVYDMDNIR VTTPKRHIDIHRGK

Data typeCount
13C chemical shifts460
15N chemical shifts161
1H chemical shifts580

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1major_E9_DNase1
2minor_E9_DNase1

Entities:

Entity 1, major_E9_DNase 134 residues - 15090 Da.

1   METGLUSERLYSARGASNLYSPROGLYLYS
2   ALATHRGLYLYSGLYLYSPROVALGLYASP
3   LYSTRPLEUASPASPALAGLYLYSASPSER
4   GLYALAPROILEPROASPARGILEALAASP
5   LYSLEUARGASPLYSGLUPHELYSSERPHE
6   ASPASPPHEARGLYSALAVALTRPGLUGLU
7   VALSERLYSASPPROGLULEUSERLYSASN
8   LEUASNPROSERASNLYSSERSERVALSER
9   LYSGLYTYRSERPROPHETHRPROLYSASN
10   GLNGLNVALGLYGLYARGLYSVALTYRGLU
11   LEUHISHISASPLYSPROILESERGLNGLY
12   GLYGLUVALTYRASPMETASPASNILEARG
13   VALTHRTHRPROLYSARGHISILEASPILE
14   HISARGGLYLYS

Samples:

sample_one: DNase domain of colicin E9, [U-99% 13C; U-99% 15N], 2.0 mM; sodium phosphate 25.0 mM; H20 90%; D20 10%; sodium azide 0.01%

sample_two: DNase domain of colicin E9, [U-99% 15N], 1.5 mM; sodium phosphate 50 mM; H20 90%; D20 10%; sodium azide 0.01%

sample_conditions_one: pH: 6.2 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablesample_conditions_one
1H-15N NOESY-HSQCnot availablenot availablesample_conditions_one
1H-15N TOCSY-HSQCnot availablenot availablesample_conditions_one
HNCOnot availablenot availablesample_conditions_one
HNCAnot availablenot availablesample_conditions_one
HN(CO)CAnot availablenot availablesample_conditions_one
CBCA(CO)NHnot availablenot availablesample_conditions_one
CBCANHnot availablenot availablesample_conditions_one
HBHA(CBCACO)NHnot availablenot availablesample_conditions_one

Software:

FELIX v95.0 - Processing triple resonance data and peak-picking. FELIX ASSIGN: initial semi-automated assignment. In-house developed user macros for data processing.

NMRPipe - Processing 3D NOESY and TOCSY and 2D HSQC

XEASY v1.2 - Defining strips for 3D NOESY to aid sequential assignment.

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker DMX 500 MHz

Related Database Links:

BMRB 4352
PDB
EMBL CAA31104 CAA33862
GB ACM07430
PRF 1615299E
REF WP_012644886 WP_044860894 YP_002533537
SP P09883