BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4295

Title: Titin Module A71 from Human Cardiac Muscle

Authors: Muhle-Goll, C.; Pastore, A.; Nilges, M.

Citation: Muhle-Goll, C.; Pastore, A.; Nilges, M.. "The 3D Structure of a Type I Module from Titin: a Prototype of Intracellular Fibronectin Type III Domains"  Structure 6, 1291-1302 (1998).

Assembly members:
Titin type I domain, polymer, 112 residues, 12458 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: not available   Kingdom: Eukaryota   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Titin type I domain: MHHHHHHSSPIDPPGKPVPL NITRHTVTLKWAKPEYTGGF KITSYIVEKRDLPNGRWLKA NFSNILENEFTVSGLTEDAA YEFRVIAKNAAGAISPPSEP SDAITCRDDVEA

Data sets:
Data typeCount
15N chemical shifts101
1H chemical shifts731

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Titin_type_I_domain-A711

Entities:

Entity 1, Titin_type_I_domain-A71 112 residues - 12458 Da.

1   METHISHISHISHISHISHISSERSERPRO
2   ILEASPPROPROGLYLYSPROVALPROLEU
3   ASNILETHRARGHISTHRVALTHRLEULYS
4   TRPALALYSPROGLUTYRTHRGLYGLYPHE
5   LYSILETHRSERTYRILEVALGLULYSARG
6   ASPLEUPROASNGLYARGTRPLEULYSALA
7   ASNPHESERASNILELEUGLUASNGLUPHE
8   THRVALSERGLYLEUTHRGLUASPALAALA
9   TYRGLUPHEARGVALILEALALYSASNALA
10   ALAGLYALAILESERPROPROSERGLUPRO
11   SERASPALAILETHRCYSARGASPASPVAL
12   GLUALA

Samples:

sample_one: Titin type I domain, [U-90% 15N], 1.2 mM; NaCl 50 mM

sample_conditions: pH: 5.6 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
HSQCnot availablenot availablenot available
3D NOESY-HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker AMX600 600 MHz

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