BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4297

Title: Chemical Shifts (1H, 13C, 15N) and J-couplings (3JHNHA, 3JNHB, 3JHAHB) for the N-terminal Domain of E. coli DnaB Helicase, DnaB(24-136)

Authors: Weigelt, Johan; Brown, Susan; Miles, Caroline; Dixon, Nicholas; Otting, Gottfried

Citation: Weigelt, Johan; Brown, Susan; Miles, Caroline; Dixon, Nicholas; Otting, Gottfried. "NMR Structure of the N-terminal Domain of E. coli DnaB Helicase: Implications for Structure Rearrangements in the Helicase Hexamer and its Biological Function"  Structure Fold Des. 7, 681-690 (1999).

Assembly members:
DnaB helicase, polymer, 114 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
DnaB helicase: MKVPPHSIEAEQSVLGGLML DNERWDDVAERVVADDFYTR PHRHIFTEMARLQESGSPID LITLAESLERQGQLDSVGGF AYLAELSKNTPSAANISAYA DIVRERAVVREMIS

Data sets:
Data typeCount
13C chemical shifts453
15N chemical shifts112
1H chemical shifts771
coupling constants190

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DnaB(24-136)1

Entities:

Entity 1, DnaB(24-136) 114 residues - Formula weight is not available

1   METLYSVALPROPROHISSERILEGLUALA
2   GLUGLNSERVALLEUGLYGLYLEUMETLEU
3   ASPASNGLUARGTRPASPASPVALALAGLU
4   ARGVALVALALAASPASPPHETYRTHRARG
5   PROHISARGHISILEPHETHRGLUMETALA
6   ARGLEUGLNGLUSERGLYSERPROILEASP
7   LEUILETHRLEUALAGLUSERLEUGLUARG
8   GLNGLYGLNLEUASPSERVALGLYGLYPHE
9   ALATYRLEUALAGLULEUSERLYSASNTHR
10   PROSERALAALAASNILESERALATYRALA
11   ASPILEVALARGGLUARGALAVALVALARG
12   GLUMETILESER

Samples:

sample_1: DnaB helicase, {U-98% 15N}, 2.2 mM; sodium phosphate 20 mM; EDTA 1 mM; sodiumn azide 0.02 % w/v

sample_2: DnaB helicase, {U-98% 13C, U-98% 15N}, 2.2 mM; sodium phosphate 20 mM; EDTA 1 mM; sodiumn azide 0.02 % w/v

sample_3: DnaB helicase, {U-10% 13C, U-98% 15N}, 2.2 mM; sodium phosphate 20 mM; EDTA 1 mM; sodiumn azide 0.02 % w/v

Ex-cond_1: pH: 7.5 na; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 4075
PDB
DBJ BAB38457 BAE78054 BAG66812 BAG79869 BAH61192
EMBL CAP78509 CAQ34401 CAQ91563 CAR01031 CAR05689
GB AAA23689 AAA23690 AAC43146 AAC77022 AAG59250
REF NP_313061 NP_418476 NP_709868 WP_000918354 WP_000918355
SP P0ACB0 P0ACB1 Q8FB22 Q8X5V3