BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4301

Title: 1H, 13C and 15N Chemical Shift Assignments of the Pathogenesis-related Protein P14a

Authors: Fernandez, Cesar; Szyperski, Thomas; Bruyere, Thierry; Ramage, Paul; Mosinger, Egon; Wuthrich, Kurt

Citation: Fernandez, Cesar; Szyperski, Thomas; Bruyere, Thierry; Ramage, Paul; Mosinger, Egon; Wuthrich, Kurt. "NMR solution structure of the pathogenesis-related protein P14a"  J. Mol. Biol. 266, 576-593 (1997).

Assembly members:
Pathogenesis-related Protein P14a, polymer, 135 residues, 14800 Da.

Natural source:   Common Name: tomato   Taxonomy ID: 4081   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Lycopersicon esculentum

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Pathogenesis-related Protein P14a: QNSPQDYLAVHNDARAQVGV GPMSWDANLASRAQNYANSR AGDCNLIHSGAGENLAKGGG DFTGRAAVQLWVSERPSYNY ATNQCVGGKKCRHYTQVVWR NSVRLGCGRARCNNGWWFIS CNYDPVGNWIGQRPY

Data sets:
Data typeCount
13C chemical shifts408
15N chemical shifts156
1H chemical shifts875

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1P14a1

Entities:

Entity 1, P14a 135 residues - 14800 Da.

1   GLNASNSERPROGLNASPTYRLEUALAVAL
2   HISASNASPALAARGALAGLNVALGLYVAL
3   GLYPROMETSERTRPASPALAASNLEUALA
4   SERARGALAGLNASNTYRALAASNSERARG
5   ALAGLYASPCYSASNLEUILEHISSERGLY
6   ALAGLYGLUASNLEUALALYSGLYGLYGLY
7   ASPPHETHRGLYARGALAALAVALGLNLEU
8   TRPVALSERGLUARGPROSERTYRASNTYR
9   ALATHRASNGLNCYSVALGLYGLYLYSLYS
10   CYSARGHISTYRTHRGLNVALVALTRPARG
11   ASNSERVALARGLEUGLYCYSGLYARGALA
12   ARGCYSASNASNGLYTRPTRPPHEILESER
13   CYSASNTYRASPPROVALGLYASNTRPILE
14   GLYGLNARGPROTYR

Samples:

sample_one: Pathogenesis-related Protein P14a 1.5 mM; H2O 90%; D2O 10%

sample_two: Pathogenesis-related Protein P14a, [U-15N], 1.5 mM; H2O 90%; D2O 10%

sample_three: Pathogenesis-related Protein P14a, [U-13C;U-15N], 1.5 mM; H2O 90%; D2O 10%

sample_conditions_p14a: pH: 5.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX600 600 MHz
  • Bruker AMX500 500 MHz
  • Varian UnityPlus 750 MHz

Related Database Links:

PDB
EMBL CAA48672 CAA70042
GB AAA03616 ABA41593
REF NP_001234314
SP P04284