BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4308

Title: 1H and 15N Chemical Shift Assignments for Domain 4 of the Common Beta-Chain of the IL-3, IL-5 and GM-CSF Receptors

Authors: Mulhern, Terrence; Booker, Grant

Citation: Mulhern, Terrence; Bagley, Christopher; Gaunt, C.; Lopez, Angel; Vadas, M.; D'Andrea, Richard; Booker, Grant. "1H and 15N Chemical Shift Assignments for Domain 4 of the Common Beta-Chain of the IL-3, IL-5 and GM-CSF Receptors"  J. Biomol. NMR 14, 281-282 (1999).

Assembly members:
beta c domain 4, polymer, 102 residues, 12096 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
beta c domain 4: MIQMAPPSLNVTKDGDSYSL RWETMKMRYEHIDHTFEIQY RKDTATWKDSKTETLQNAHS MALPALEPSTRYWARVRVRT SRTGYNGIWSEWSEARSWDT ES

Data sets:
Data typeCount
15N chemical shifts111
1H chemical shifts710

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta c domain 41

Entities:

Entity 1, beta c domain 4 102 residues - 12096 Da.

1   METILEGLNMETALAPROPROSERLEUASN
2   VALTHRLYSASPGLYASPSERTYRSERLEU
3   ARGTRPGLUTHRMETLYSMETARGTYRGLU
4   HISILEASPHISTHRPHEGLUILEGLNTYR
5   ARGLYSASPTHRALATHRTRPLYSASPSER
6   LYSTHRGLUTHRLEUGLNASNALAHISSER
7   METALALEUPROALALEUGLUPROSERTHR
8   ARGTYRTRPALAARGVALARGVALARGTHR
9   SERARGTHRGLYTYRASNGLYILETRPSER
10   GLUTRPSERGLUALAARGSERTRPASPTHR
11   GLUSER

Samples:

sample_1: beta c domain 4, [U-99% 15N], 0.7 mM; phosphate 10 mM; D20 10%; H2O 90%

sample_2: beta c domain 4, [U-99% 15N], 0.7 mM; phosphate 10 mM; D2O 100%

Ex-cond_1: pH: 6.1 na; temperature: 293 K

Ex-cond_2: pH*: 6.1 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
1H-15N HMQC-Jnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available
1H-15N TOCSY-HSQCnot availablenot availablenot available

Software:

XEASY - assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAG10649 BAG64130
EMBL CAG30314 CAK54420 CAK54719
GB AAA18171 AAB31055 AAH70085 EAW60125 EAW60126
REF NP_000386 XP_003821586 XP_003821587 XP_004063464 XP_004063465
SP P32927