BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4317

Title: 1H, 13C, and 15N Chemical Shift Assignments for NS1(1-73)

Authors: Chien, Chen-ya; Montelione, Gaetano

Citation: Chien, Chen-ya; Tejero, R.; Huang, Y.; Zimmerman, D.; Rios, C.; Krug, R.; Montelione, Gaetano. "A novel RNA-binding motif in influenza A virus non-structural protein 1"  Nat. Struct. Biol. 4, 891-. (1997).

Assembly members:
Non-structural protein 1, polymer, 73 residues, 8300 Da.

Natural source:   Common Name: Flu Virus   Taxonomy ID: 11320   Superkingdom: Viruses   Kingdom: not available   Genus/species: Influenza virus A and B group Influenza A Virus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Non-structural protein 1: MDSNTVSSFQVDCFLWHVRK QVVDQELGDAPFLDRLRRDQ KSLRGRGSTLGLNIEAATHV GKQIVEKILKEES

Data sets:
Data typeCount
13C chemical shifts303
15N chemical shifts85
1H chemical shifts534

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NS1(1-73) subunit 11
2NS1(1-73) subunit 21

Entities:

Entity 1, NS1(1-73) subunit 1 73 residues - 8300 Da.

1   METASPSERASNTHRVALSERSERPHEGLN
2   VALASPCYSPHELEUTRPHISVALARGLYS
3   GLNVALVALASPGLNGLULEUGLYASPALA
4   PROPHELEUASPARGLEUARGARGASPGLN
5   LYSSERLEUARGGLYARGGLYSERTHRLEU
6   GLYLEUASNILEGLUALAALATHRHISVAL
7   GLYLYSGLNILEVALGLULYSILELEULYS
8   GLUGLUSER

Samples:

sample_1: NS1(1-73) dimer 2.0 mM; NH4)Ac 50 mM; D2O 5%; H2O 95%

sample_2: NS1(1-73) dimer, [U-100% 15N], 2.5 mM; NH4)Ac 50 mM; D2O 5%; H2O 95%

sample_3: NS1(1-73) dimer, [U-100% 15N; U-100% 13C], 2.0 mM; NH4)Ac 50 mM; D2O 5%; H2O 95%

condition_1: pH: 6.0 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablecondition_1
HNCOnot availablenot availablecondition_1
CANHnot availablenot availablecondition_1
CA(CO)NHnot availablenot availablecondition_1
H(CA)NHnot availablenot availablecondition_1
H(CA)(CO)NHnot availablenot availablecondition_1
CBCANHnot availablenot availablecondition_1
CBCA(CO)NHnot availablenot availablecondition_1
HCC(CO)NH-TOCSYnot availablenot availablecondition_1
HCCH-COSYnot availablenot availablecondition_1
4 channel probe with pulse field gradientnot availablenot availablecondition_1

Software:

vnmr v5.3 - Data processing

NMRcompass - Spectra analysis and peakpicking

AUTOASSIGN - 1H, 13C, 15N resonance assignment, Software developed in Montelione lab [Zimmerman et al. (1997) J. Mol. Biol. 269:592-610] for automating the peak assignment

NMR spectrometers:

  • Varian . 500 MHz
  • Varian . 600 MHz

Related Database Links:

BMRB 15117
PDB
DBJ BAA01428
EMBL CAA24288 CAC09426
GB AAA43086 AAA43515 AAA43688 AAC36136 AAC36138
PRF 2005320B 2005320D
SP P03494 P03495 P69277 P69278 Q1PUD3