BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4326

Title: Complete 1H, 13C, and 15N Chemical Shift Assignments for the N-terminal Domain of DNA Polymerase B (residues 2-87).

Authors: Liu, Dingjiang; DeRose, Eugene; Prasad, Rajendra; Wilson, Samuel; Mullen, Gregory

Citation: Liu, Dingjiang; DeRose, Eugene; Prasad, Rajendra; Wilson, Samuel; Mullen, Gregory. "Assignments of 1H, 15N, and 13C Resonances for the Backbone and Side Chains of the N-Terminal Domain of DNA Polymerase B. Determination of the Secondary Structure and Tertiary Contacts"  Biochemistry 33, 9537-9545 (1994).

Assembly members:
DNA Polymerase B, polymer, 87 residues, 9490 Da.

Natural source:   Common Name: rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DNA Polymerase B: MSKRKAPQETLNGGITDMLV ELANFEKNVSQAIHKYNAYR KAASVIAKYPHKIKSGAEAK KLPGVGTKIAEKIDEFLATG KLRKLEK

Data sets:
Data typeCount
13C chemical shifts312
15N chemical shifts83
1H chemical shifts598

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1B-Pol N-terminal Domain1

Entities:

Entity 1, B-Pol N-terminal Domain 87 residues - 9490 Da.

1   METSERLYSARGLYSALAPROGLNGLUTHR
2   LEUASNGLYGLYILETHRASPMETLEUVAL
3   GLULEUALAASNPHEGLULYSASNVALSER
4   GLNALAILEHISLYSTYRASNALATYRARG
5   LYSALAALASERVALILEALALYSTYRPRO
6   HISLYSILELYSSERGLYALAGLUALALYS
7   LYSLEUPROGLYVALGLYTHRLYSILEALA
8   GLULYSILEASPGLUPHELEUALATHRGLY
9   LYSLEUARGLYSLEUGLULYS

Samples:

sample_one: DNA Polymerase B, [U-100% 13C; U-100% 15N], 2.8 mM; Tris-d11 5 mM; NaCl 400 mM

sample_two: DNA Polymerase B, [U-100% 15N], 4.7 mM; Tris-d11 5 mM; NaCl 400 mM

sample_three: DNA Polymerase B 1.4 mM; Tris-d11 5 mM; NaCl 400 mM

sample_conditions: ionic strength: 0.800 .; pH: 6.7 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYnot availablenot availablesample_conditions
2D 1H-1H TOCSYnot availablenot availablesample_conditions
2D 1H-1H DQF-COSYnot availablenot availablesample_conditions
2D 1H-15N HMQCnot availablenot availablesample_conditions
2D 1H-15N HMQC-Jnot availablenot availablesample_conditions
3D 1H-15N-1H NOESY-HMQCnot availablenot availablesample_conditions
3D 1H-15N-1H TOCSY-HMQCnot availablenot availablesample_conditions
3D 1H-13C-1H TOCSY-HMQCnot availablenot availablesample_conditions
3D HNCAnot availablenot availablesample_conditions
3D HN(CO)CAnot availablenot availablesample_conditions
3D HNCOnot availablenot availablesample_conditions
3D 1H-13C-1H NOESY-HMQCnot availablenot availablesample_conditions

Software:

Felix v2.10 - processing and analysis of spectra

NMR spectrometers:

  • General Electric GN-500 500 MHz

Related Database Links:

BMRB 18267
PDB
DBJ BAA06099 BAC36630 BAD97257 BAE27405 BAE30399
EMBL CAD29079 CAD29080 CAD29081 CAD29082 CAD29083
GB AAA41900 AAA41901 AAA60133 AAB00389 AAB59441
REF NP_001029936 NP_001244632 NP_001297194 NP_002681 NP_035260
SP P06746 P06766 Q27958 Q8K409
TPG DAA14453