BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4327

Title: 1H, 13C, and 15N Resonance Assignment and Secondary Structure of the N-terminal Inhibitory Domain of Human Tissue Inhibitor of Metalloproteinases-1

Authors: Wu, Bin; Arumugam, S.; Huang, Wen; Brew, Keith; Van Doren, Steven

Citation: Wu, Bin; Arumugam, S.; Huang, Wen; Brew, Keith; Van Doren, Steven. "1H, 13C and 15N Resonance Assignment and Secondary Structure of the N-terminal Inhibitory Domain of Human Tissue Inhibitor of Metalloproteinases-1"  J. Biomol. NMR 14, 289-290 (1999).

Assembly members:
N-terminal inhibitory domain of human tissue inhibitor of metalloproteinases-1, polymer, 126 residues, 14236 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
N-terminal inhibitory domain of human tissue inhibitor of metalloproteinases-1: CTCVPPHPQTAFCNSDLVIR AKFVGTPEVNQTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVCGYFHRSHNRS EEFLIAGKLQDGLLHITTCS FVAPWNSLSLAQRRGFTKTY TVGCEE

Data sets:
Data typeCount
13C chemical shifts539
15N chemical shifts122
1H chemical shifts845

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1N-TIMP-11

Entities:

Entity 1, N-TIMP-1 126 residues - 14236 Da.

1   CYSTHRCYSVALPROPROHISPROGLNTHR
2   ALAPHECYSASNSERASPLEUVALILEARG
3   ALALYSPHEVALGLYTHRPROGLUVALASN
4   GLNTHRTHRLEUTYRGLNARGTYRGLUILE
5   LYSMETTHRLYSMETTYRLYSGLYPHEGLN
6   ALALEUGLYASPALAALAASPILEARGPHE
7   VALTYRTHRPROALAMETGLUSERVALCYS
8   GLYTYRPHEHISARGSERHISASNARGSER
9   GLUGLUPHELEUILEALAGLYLYSLEUGLN
10   ASPGLYLEULEUHISILETHRTHRCYSSER
11   PHEVALALAPROTRPASNSERLEUSERLEU
12   ALAGLNARGARGGLYPHETHRLYSTHRTYR
13   THRVALGLYCYSGLUGLU

Samples:

15N-labeled_N-TIMP-1: N-terminal inhibitory domain of human tissue inhibitor of metalloproteinases-1, [U-15N], 0.5 – 0.6 mM; sodium acetate d3 20 mM; NaCl 150 mM; NaN3 1 mM; D2O 7%; H2O 93%

13C_15N-labeled_N-TIMP-1: N-terminal inhibitory domain of human tissue inhibitor of metalloproteinases-1, [U-13C; U-15N], 0.9 – 1.0 mM; sodium acetate d3 20 mM; NaCl 150 mM; NaN3 1 mM; D2O 7%; H2O 93%

16%_13C-labeled_N-TIMP-1: N-terminal inhibitory domain of human tissue inhibitor of metalloproteinases-1, [U-16% 13C], 0.4 – 0.5 mM; sodium acetate d3 20 mM; NaCl 150 mM; NaN3 1 mM; D2O 7%; H2O 93%

standard_conditions: ionic strength: 0.171 .; pH: 6.0 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablestandard_conditions
HNCAnot availablenot availablestandard_conditions
(HA)CA(CO)NHnot availablenot availablestandard_conditions
HN(CA)HAnot availablenot availablestandard_conditions
HN(CACO)NHnot availablenot availablestandard_conditions
HNCOnot availablenot availablestandard_conditions
CBCA(CO)NHnot availablenot availablestandard_conditions
CCA(CO)NHnot availablenot availablestandard_conditions
HCCH-TOCSYnot availablenot availablestandard_conditions
15N NOESY-HSQCnot availablenot availablestandard_conditions
time-shared NOESY-(13C,15N)-HSQCnot availablenot availablestandard_conditions
13C HSMQC-NOESYnot availablenot availablestandard_conditions
13C HSQCnot availablenot availablestandard_conditions
Nalorac 8 mm triple resonance probe with shiedednot availablenot availablestandard_conditions
z-gradient coilnot availablenot availablestandard_conditions

Software:

SYBYL TRIAD v6.2 or 6.3 - process and interpret NMR spectra

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAA01913 BAG34878 BAG52016
EMBL CAA26443 CAA26902 CAG28566 CAG46779 CAH90650
GB AAA52436 AAA63234 AAA99943 AAD14009 AAH00866
PIR JC4303
PRF 1107278A 1308125A
REF NP_001028111 NP_001125351 NP_003245 XP_003917687 XP_005593482
SP P01033 P49061 Q5RC60 Q95KL9