BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4336

Title: Backbone and Sequence-Specific Assignment of Three Forms of the Lipoate Containing H-protein of the Glycine Decarboxylase Complex.   PubMed: 10605092

Authors: Guilhaudis, Laure; Simorre, Jean-Pierre; Bouchayer, Eftychia; Neuburger, Michel; Bourguignon, Jacques; Douce, Roland; Marion, Dominique; Gans, Pierre

Citation: Guilhaudis, Laure; Simorre, Jean-Pierre; Bouchayer, Eftychia; Neuburger, Michel; Bourguignon, Jacques; Douce, Roland; Marion, Dominique; Gans, Pierre. "Backbone and Sequence-Specific Assignment of Three Forms of the Lipoate Containing H-protein of the Glycine Decarboxylase Complex."  J. Biomol. NMR 15, 185-186 (1999).

Assembly members:
Glycine Cleavage System H-protein, polymer, 131 residues, 13948 Da.
S-aminomethyldihydrolipoate, non-polymer, Formula weight is not available

Natural source:   Common Name: Garden Pea   Taxonomy ID: 3888   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Pisum sativum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Glycine Cleavage System H-protein: SNVLDGLKYAPSHEWVKHEG SVATIGITDHAQDHLGEVVF VELPEPGVSVTKGKGFGAVE SVKATSDVNSPISGEVIEVN TGLTGKPGLINSSPYEDGWM IKIKPTSPDELESLLGAKEY TKFCEEEDAAH

Data sets:
Data typeCount
13C chemical shifts413
15N chemical shifts129
1H chemical shifts840

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hmet subunit 11
2S-aminomethyldihydrolipoate2

Entities:

Entity 1, Hmet subunit 1 131 residues - 13948 Da.

1   SERASNVALLEUASPGLYLEULYSTYRALA
2   PROSERHISGLUTRPVALLYSHISGLUGLY
3   SERVALALATHRILEGLYILETHRASPHIS
4   ALAGLNASPHISLEUGLYGLUVALVALPHE
5   VALGLULEUPROGLUPROGLYVALSERVAL
6   THRLYSGLYLYSGLYPHEGLYALAVALGLU
7   SERVALLYSALATHRSERASPVALASNSER
8   PROILESERGLYGLUVALILEGLUVALASN
9   THRGLYLEUTHRGLYLYSPROGLYLEUILE
10   ASNSERSERPROTYRGLUASPGLYTRPMET
11   ILELYSILELYSPROTHRSERPROASPGLU
12   LEUGLUSERLEULEUGLYALALYSGLUTYR
13   THRLYSPHECYSGLUGLUGLUASPALAALA
14   HIS

Entity 2, S-aminomethyldihydrolipoate 1 residues - . - Formula weight is not available

1   S-AMDHP

Samples:

sample_one: Glycine Cleavage System H-protein, [U-99% 15N], 2.0 mM; potassium phosphate 50 mM; EDTA 0.1 mM; NaN3 0.2%; D2O 10%; H2O 90%

sample_two: Glycine Cleavage System H-protein, [U-99% 13C; U-99% 15N], 2.0 mM; potassium phosphate 50 mM; EDTA 0.1 mM; NaN3 0.2%; D2O 10%; H2O 90%

sample_conditions: pH: 5.5 na; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESY-HSQCnot availablenot availablesample_conditions
3D 1H-15N TOCSY-HSQCnot availablenot availablesample_conditions
3D HNCAnot availablenot availablesample_conditions
3D HCCH-TOCSYnot availablenot availablesample_conditions
2D 1H-15N HSQCnot availablenot availablesample_conditions
2D 1H-13C HSQCnot availablenot availablesample_conditions
2D 1H-1H NOESYnot availablenot availablesample_conditions

Software:

FELIX v95.0; 97.0 -

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Varian INOVA 400 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 4337 4338
PDB
EMBL CAA37704 CAA45978 CAI79404 CAJ13415 CAJ13721
GB AAA33668
PRF 1923203A
SP P16048