BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4341

Title: Assignment of the Backbone Resonances of Oxidized Fe-superoxide Dismutase, a 42 kDa Paramagnet-containing Enzyme

Authors: Vathyam, Surekha; Byrd, R.; Miller, Anne-Frances

Citation: Vathyam, Surekha; Byrd, R.; Miller, Anne-Frances. "Assignment of the Backbone Resonances of Oxidized Fe-superoxide Dismutase, a 42 kDa Paramagnet-containing Enzyme"  J. Biomol. NMR 14, 293-294 (1999).

Assembly members:
Fe-superoxide dismutase, polymer, 192 residues, 21000 Da.
FE (III) ION, non-polymer, 55.845 Da.
WATER, water, 18.015 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Fe-superoxide dismutase: SFELPALPYAKDALAPHISA ETIEYHYGKHHQTYVTNLNN LIKGTAFEGKSLEEIIRSSE GGVFNNAAQVWNHWFYWNCL APNAGGEPTGKVAEAIAASF GSFADFKAQFTDAAIKNFGS GWTWLVKNSDGKLAIVSTSN AGTPLTTDATPLLTVDVWEH AYYIDYRNARPGYLEHFWAL VNWEFVAKNLAA

Data sets:
Data typeCount
13C chemical shifts381
15N chemical shifts117
1H chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fe(II)SOD monomer 11
2Fe(II)SOD monomer 21
3Fe(II) monomer 12
4Fe(II) monomer 22
5H2O monomer 13
6H2O monomer 23

Entities:

Entity 1, Fe(II)SOD monomer 1 192 residues - 21000 Da.

1   SERPHEGLULEUPROALALEUPROTYRALA
2   LYSASPALALEUALAPROHISILESERALA
3   GLUTHRILEGLUTYRHISTYRGLYLYSHIS
4   HISGLNTHRTYRVALTHRASNLEUASNASN
5   LEUILELYSGLYTHRALAPHEGLUGLYLYS
6   SERLEUGLUGLUILEILEARGSERSERGLU
7   GLYGLYVALPHEASNASNALAALAGLNVAL
8   TRPASNHISTRPPHETYRTRPASNCYSLEU
9   ALAPROASNALAGLYGLYGLUPROTHRGLY
10   LYSVALALAGLUALAILEALAALASERPHE
11   GLYSERPHEALAASPPHELYSALAGLNPHE
12   THRASPALAALAILELYSASNPHEGLYSER
13   GLYTRPTHRTRPLEUVALLYSASNSERASP
14   GLYLYSLEUALAILEVALSERTHRSERASN
15   ALAGLYTHRPROLEUTHRTHRASPALATHR
16   PROLEULEUTHRVALASPVALTRPGLUHIS
17   ALATYRTYRILEASPTYRARGASNALAARG
18   PROGLYTYRLEUGLUHISPHETRPALALEU
19   VALASNTRPGLUPHEVALALALYSASNLEU
20   ALAALA

Entity 2, Fe(II) monomer 1 - Fe - 55.845 Da.

1   FE

Entity 3, H2O monomer 1 - 18.015 Da.

1   HOH

Samples:

sample_1: Fe-superoxide dismutase, [U-99% 2H, U-99% 13C, U-99% 15N], 1.57 mM

Ex-cond_1: pH: 6.46 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
HN(CA)CBsample_1not availableEx-cond_1
HN(COCA)CBsample_1not availableEx-cond_1
HN(CO)CACBsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HSQCsample_1not availableEx-cond_1

Software:

nmrPipe v1.6 - processed NMR data

ANSIG v3.3 - made peak assignments

NMR spectrometers:

  • Varian Unity-Plus 500 MHz

Related Database Links:

BMRB 4315
PDB
DBJ BAA15422 BAA84485 BAA84490 BAB35788 BAG77304
EMBL CAP76155 CAQ32133 CAQ88908 CAQ98565 CAR03017
GB AAA24637 AAC74728 AAG56645 AAN43263 AAN80510
REF NP_310392 NP_416173 NP_707556 WP_000007270 WP_000007272
SP P0AGD3 P0AGD4 P0AGD5 P0AGD6